Pharmacology & Pharmacy

Volume 9, Issue 6 (June 2018)

ISSN Print: 2157-9423   ISSN Online: 2157-9431

Google-based Impact Factor: 0.70  Citations  h5-index & Ranking

Uromedic® Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor in Vitro

HTML  XML Download Download as PDF (Size: 683KB)  PP. 193-207  
DOI: 10.4236/pp.2018.96015    1,438 Downloads   4,517 Views  Citations

ABSTRACT

Dihydrotestosterone (DHT) is implicated in the development of benign prostate hyperplasia (BPH). We investigated if Uromedic? pumpkin (variety of Cucurbita pepo L. convar. citrullinina GREB. var. styriaca GREB) seed soft extract (active ingredients of GRANUFINK? Prosta forte 500 mg), seed oil and isolated Δ7-sterols could inhibit the conversion of [1,2,6,7-3H(N)]-testosterone to DHT by 5α-reductases. Also, we tested competition with [3H]-DHT for binding to the androgen receptor (AR). Pumpkin seed oil and pumpkin seed soft extract were identified as moderately active inhibitors of 5α-R1 and 5α-R2, with almost similar inhibitory capacities (IC50 < 5 mg/ml for 5α-R1 and about IC50 = 6 mg/ml for 5α-R2). The isolated Δ7-sterols were more active inhibitors (IC50 = 0.3 mg/ml for 5α-R1, IC50 = 1.0 mg/ml for 5α-R2). All three test compounds bound to the AR dose-dependently, with strong binding by Δ7-sterols (IC50 = 0.2 mg/ml) and weaker binding by pumpkin seed oil (IC50 = 0.4 mg/ml) and pumpkin seed soft extract (IC50 = 1.1 mg/ml). We propose that inhibition of 5α-reductases and competitive binding to the AR are mechanisms of action, by which the Uromedic? pumpkin seed derived test compounds, most specifically Δ7-sterols, counteract DHT and thereby exert clinically positive effects on the prostate, as well as bladder-strengthening effects.

Share and Cite:

Heim, S. , Seibt, S. , Stier, H. and Moré, M. (2018) Uromedic® Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor in Vitro. Pharmacology & Pharmacy, 9, 193-207. doi: 10.4236/pp.2018.96015.

Cited by

[1] Selective Supercritical CO2 Extraction and Biocatalytic Valorization of Cucurbita pepo L. Industrial Residuals
Molecules, 2022
[2] Extract from Cucurbita pepo improves BPH symptoms without affecting sexual function: a 24-month noninterventional study
World Journal of …, 2022
[3] Plantas medicinais com ação na hiperplasia benigna da próstata
2021
[4] Characterization of lipid fraction of Apiaceae family seed spices: Impact of species and extraction method
2021
[5] Neuroprotective effect of wild lowbush blueberry (Vaccinium angustifolium) on global cerebral ischemia/reperfusion injury in rats: Downregulation of iNOS/TNF‐α and …
Phytotherapy …, 2021
[6] The Potential of Pumpkin Seeds as a Functional Food Ingredient: A Review
2020
[7] Seed Lipid Composition of New Hybrids of Styrian Oil Pumpkin Grown in Poland
2020
[8] Effects of vermicompost, bio-nitrogen (Barvar 1), and bio-phosphorus (Barvar 2) fertilizers on the yield and yield components of medical pumpkin (Cucurbita pepo L …
2020
[9] Scientific African
2020
[10] Cucurbita pepo–Rhus aromatica–Humulus lupulus Combination Reduces Overactive Bladder Symptoms in Women–A Noninterventional Study
2019
[11] Attenuation of Benign Prostatic Hyperplasia by Optimized Tadalafil Loaded Pumpkin Seed Oil-Based Self Nanoemulsion: In Vitro and In Vivo Evaluation
2019
[12] Application of cryogenic grinding pretreatment to enhance extractability of bioactive molecules from pumpkin seed cake
2019

Journals Menu
Follow SCIRP
Contact us
+1 323-425-8868
customer@scirp.org
WhatsApp +86 18163351462(WhatsApp)
Click here to send a message to me 1655362766
Paper Publishing WeChat

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.