Authentication and Genetic Origin of Medicinal Angelica acutiloba Using Random Amplified Polymorphic DNA Analysis - American Journal of Plant Sciences

AJPS > Vol.4 No.2, February 2013

American Journal of Plant Sciences

Volume 4, Issue 2 (February 2013)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

Authentication and Genetic Origin of Medicinal Angelica acutiloba Using Random Amplified Polymorphic DNA Analysis ()

HTML XML

Download as PDF (Size: 349KB) PP. 269-273

DOI: 10.4236/ajps.2013.42035 4,856 Downloads 6,795 Views Citations

Author(s)

Kiyoshi Matsubara, Satoshi Shindo, Hitoshi Watanabe, Fumio Ikegami

Affiliation(s)

Center for Environment, Health and Field Sciences, Chiba University, Kashiwa, Japan.

ABSTRACT

Some Angelica species are used for medicinal purposes. In particular, the roots of Angelica acutiloba var. acutiloba and A. acutiloba var. sugiyamae, known as “Toki” and “Hokkai Toki”, respectively, are used as important medicinal materials in traditional Japanese medicine. However, since these varieties have recently outcrossed with each other, it is difficult to determine whether the Japanese Angelica Root material used as a crude drug is the “pure” variety. In this study, we developed an efficient method to authenticate A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae from each other and from other Angelica species/varieties. The random amplified polymorphic DNA (RAPD) method efficiently discriminated each Angelica variety. A. acutiloba var. sugiyamae was identified via a characteristic fragment amplified by the decamer primer OPD-15. This fragment showed polymorphisms among Angelica species/varieties. The unique fragment derived from A. acutiloba var. sugiyamae was also found in one strain of A. acutiloba var. acutiloba, implying that this strain arose from outcrossing between A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae. This RAPD marker technique will be useful for practical and accurate authentication among A. acutiloba var. acutiloba, A. acutiloba var. sugiyamae, and their adulterants.

KEYWORDS

Angelica acutiloba; Intraspecific Variation; Kampo Medicine; Random Amplified Polymorphic DNA (RAPD)

Share and Cite:

Matsubara, K. , Shindo, S. , Watanabe, H. and Ikegami, F. (2013) Authentication and Genetic Origin of Medicinal Angelica acutiloba Using Random Amplified Polymorphic DNA Analysis. American Journal of Plant Sciences, 4, 269-273. doi: 10.4236/ajps.2013.42035.

Cited by

[1]	Current State and Prospects of DNA Barcoding and DNA Fingerprinting in the Analysis of the Quality of Plant Raw Materials and Plant-Derived Drugs
	2019

[2]	Современное состояние и перспективы использования ДНК-штрихкодирования и ДНК-фингерпринтинга для анализа качества лекарственного …
	2019

[3]	A comprehensive study on genetic diversity among economically important medicinal plant B. monnieri using RAPD-PCR technique
	2016

[4]	Plant Gene
	2015

[5]	Authentication of medicinal plants by DNA markers
	Plant Gene, 2015

[6]	Authentication of Silybum marianum Varieties Using RAPD Analysis
	Plant Tissue Culture and Biotechnology, 2014

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies