J. Biomedical Science and Engineering, 2009, 2, 20-23
Published Online February 2009 in SciRes. http://www.scirp.org/journal/jbise JBiSE
Researches on a practical system for concentration
detection of human exhaled CO2 gas
Rong-Guo Yan1, Bin Ge1, Hai-Ming Xie1
School of medical instrument and food engineering, University of Shanghai for Science and Technology, Shanghai 200093. Correspondence should be ad-
dressed to Rong-Guo Yan (yanrongguo@usst.edu.cn), Tel: +86 21 55271115.
Received July 30th, 2008; revised November 12th, 2008; accepted November 19th, 2008
In clinics, especially in the emergency depart-
ment, carbon dioxide (CO2) is considered as the
sixth vital sign in evaluating a patient’s medical
status. However, its wide applications in devel-
oping countries are dissatisfactory due to their
high cost compared to their economic growth.
In order to develop a practical system for con-
centration detection of human exhaled CO2 gas
for our own, we studied the detection principle
based upon the non-dispersive infrared (NDIR)
measuring technique and related Lambert-
Beer’s equation carefully, gave out our func-
tional circuit design of the system, and provided
the corresponding graphical user interface (GUI)
for CO2 detection. Finally, the experiment shows
that it may be a practical system, and will give
benefits for the development of our medical care
in the future.
Keywords: CO2 Concentration Detection, NDIR,
Lambert-Beer’s Equation, Detection Module,
Instrument Module
As we know, carbon dioxide (CO2), as a gas byproduct
produced by human cellular metabolism and finally ex-
haled out into the air through our noise, can be measured
to reflect the systematic functions of metabolism, circu-
lation and ventilation [1,2,3]. Especially, through the
noninvasive monitoring of end-tidal carbon dioxide
(ETCO2), which is also known as capnography measur-
ing the maximum carbon dioxide level reached at the end
of each breath [4,5,6], it can provide the doctor or the
nurse the sixth vital sign, as important as five other signs,
i.e., respiration, heart rate, temperature, blood pressure
and pain in evaluating the medical status of the subjects.
In America, capnography, other than monitoring pulse
oxygen saturation, has already been considered as one of
the “basic standards” for monitoring for all the subjects
receiving anesthesia.
The most common principles for CO2 detection are
based upon non-dispersive infrared (NDIR) measure-
ment or based upon chemical reactions. Commonly,
chemical CO2 gas sensors with sensitive layers have the
principal advantage of very low energy consumption and
can be reduced in size to fit into microelectronic based
systems. However, compared with the NDIR measure-
ment principle [7], chemical CO2 sensors face major
obstacles like short-and long term drift effects and a
rather low overall lifetime [8,9].
Although the technology carried out for monitoring
expired CO2 gas based upon NDIR is not a new tech-
nology [10,11,12], its wide applications, such as in me-
chanical ventilator, anesthesia machine, and etc., in de-
veloping countries are still left in a dissatisfactory condi-
tion due to its high cost compared to their economic
growth. However nowadays, with the development of
integrated circuits and non-dispersive infrared (NDIR)
sensing technology, its utilization by clinicians outside
the operating room or anesthesia realm has also grown,
especially in the emergency department.
Thus, this paper will present the principle for CO2 gas
concentration measurement; introduce its functional cir-
cuit design that we applied; and provide its current ex-
perimental result to show its practicability for use.
The method we used for concentration detection of CO2
gas is based upon the NDIR measuring technique and a
so-called well-known Lambert-Beer’s equation [13]. The
equation is given as follow:
∗= 0 (1)
I0 = the intensity of light incident on the sample;
I = the measured intensity of light after the sample;
k = the absorption coefficient of the analyte gas at the
characteristic wavelength (cm2);
c = the concentration of the analyte gas (1/cm3);
l = the path-length or the distance that the light trav-
erses the sample gas (cm).
The project is supported by the research award fund for selectively
culturing excellent young teachers in higher educations in shanghai
(grant No. slg-07045).
SciRes Copyright © 2009
R. G. Yan et al. / J. Biomedical Science and Engineering 2 (2009) 20-23 21
SciRes Copyright © 2009 JBiSE
A−= (2)
Then according to equation (1), there exists,
kclA = (3)
In the equation (3), A is expressed in terms of an ab-
sorbance. As we know, the parameter l is already deter-
mined when the detection system is designed, and the pa-
rameters A and k can be measured in the experiment, then
CO2 gas concentration c can be calculated accordingly.
As Figure 1 shows, the chamber is made of aluminum;
the left-side inner surface adopts arc shape, being spe-
cially polished and shot-blasted to increase the intensity
onto the right-side detector.
And, basically the concentration detection system for
CO2 gas consists of the following four components in-
cluding: (1) a pulsed infrared (IR) light source at the left
end of the chamber or sample room, whose excitation
frequency is about 1Hz to provide a non-dispersive infra-
red flashing source; (2) an airway chamber having l path
length with a gas inlet and a gas outlet at the up side edge
as Figure 1 shows. The gas inlet is connected to our nose
with a nose-oxygen-tube like pipe, while the gas outlet
gives a way for CO2 gas flowing out of the chamber, and
the chamber will be full of CO2 gas when performing de-
tection; and (3) an IR detector at the right end of the
chamber. The detector is in fact a dual channel PerkinEl-
mer thermopile sensor housing with two window openings
(channel T1 and T2)-each window specialized to be
equipped with band-pass filters for gas detection [14]. One
optical window opening (T2) senses light at a specific
wavelength of 4.26μm, a predominant absorption band of
CO2 gas (see Figure 2), while the other channel (T1) actu-
ally carries a band-pass wavelength centered at 4.0μm,
other than CO2 gas absorption band at 4.26μm, acting as
reference [14]. Thus except ground signal, three other sig-
nals that the thermopile detector gives are a reference sig-
nal (Ref_sig. in Figure 1) for reference use, a real meas-
ured signal (Msd_sig. in Figure 1), and a temperature sig-
nal (Tmp_sig. in Figure 1) giving the real temperature
inside the thermopile sensor for later temperature compen-
sation use. Basically, these millivolts (mV) level signals
should be amplified, conditioned via a detection module,
and then the actual CO2 concentration can be calculated
according to the Lambert-Beer’s equation by the MCU in
an instrument module. Functions of the detection module
and the instrument module are described in section 3.
Figure 1. NDIR optical detection system
891012 14 16
Intensity of transmitted radiation
Figure 2. Optical spectral absorption bands of
CO2, 4.26μm is its predominant absorption peak,
and the IR light in between this narrowband can
pass the interference filter
To accomplish CO2 gas concentration detection as the
principle indicates, we designed a practical detection
system including two functional modules: the detection
module and the instrument module.
3.1. The Detection Module
The detection module performed signal amplification,
conditioning and voltage following of four signals that
are needed to estimate gas concentration of exhaled CO2
as the Lambert-Beer’s equation indicates, including one
room temperature signal (Room_sig.) and three other
signals from the thermopile detector, i.e., a reference
signal (Ref_sig.), a real measured gas concentration
output (Msd_sig.) and a temperature output (Tmp_sig.)
for later temperature compensation (See section 2). Fi-
nally, these four signals will be provided for the instru-
ment module for calculation.
3.2. The Instrument Module
The instrument module mainly performs (1) analog-to-
digital (A/D) conversion of four signals including a
Room_sig., a Ref_sig., a Msd_sig., and a Tmp_sig. from
the detection module; (2) calculation gas concentration
according to the Lamber-Beer’s equation; (3) concentra-
tion display on the liquid crystal display (LCD) of the
acquired CO2 gas at the sample time; (4) giving warnings
and alarms; (5) configuration by a master PC using a
RS-232C universal serial interface. Thus, the main
components in the instrument module are: (1) an AT-
mega16L micro controller unit (MCU), which is a
high-performance, low-power AVR® 8-bit microcontroller
with an advanced reduced instruction set computer
(RISC) architecture and 16KB in-system programmable
flash program memory [15]. The MCU is good for pro-
gramming and debugging using C language; (2) a master
PC performing to finish configuration of the system,
such as serial number information, warning limits (upper
and lower limit) of gas concentration, calibration and
temperature compensation information, and etc., through
the RS-232C interface; (3) a DS1307 real time clock
(RTC), which is a low-power, full binary-coded decimal
22 R. G. Yan et al. / J. Biomedical Science and Engineering 2 (2009) 20-23
SciRes Copyright © 2009 JBiSE
clock/calendar. Address and data are transferred serially
through a two-wire interface (TWI), bidirectional bus
that Atmega16L provides. The current time can be set
through the RS-232C based graphical user interface
written in computer advanced languages, Visual C++,
MATLAB, and etc., for example; (4) a beeper and a
HF12232F-based LCD, which dynamically gives current
time, gas concentration information at current time, and
warnings, as well as other information that can be visu-
ally prompted on the LCD. The LCD interfaces with the
MCU through the serial peripheral interface (SPI) interface
that the Atmeag16L provides. (5) an A/D conversion of
four signals from the detection module, including one room
temperature signal (Room_sig.) and three signals from the
thermopile, i.e., a reference signal (Ref_sig.), a real meas-
ured output (Msd_sig.) and a temperature output (Tmp_sig.)
for later temperature compensation use. The functions of the
instrument module can be seen in Figure 3.
Figure 4 is a graphical user interface (GUI) for CO2
concentration detection, which is written in MATLAB.
MATLAB is selected in the paper for its simple pro-
gramming, rich graphic facilities, built-in functions, and
extensive toolboxes. This is especially suitable for engi-
neering professionals like us. From the GUI, we can se-
lect serial port (COM1, COM2, and etc.) and baud rate
(9600bps, 115200bps, and etc.) to establish communica-
tions between the master PC and the Atmeaga16L MCU
to perform gas concentration detections using the button
SendFF, where hexadecimal 0xFF is used as a hand-
shaking string between two CPUs. Moreover, the button
Load Data can load any related saved data. In the
graphical window, the real measured output (corre-
sponding to Msd_sig.) and its reference output (corre-
sponding to Ref_sig.) in an experimental test are plotted
with different signs. Their minimal value, maximal value,
and averaged peak-to-peak difference in the sample pe-
riod are given in the right frame box.
In order to eliminate errors from intensity variation of
the light, and to decrease sensor drift due to temperature
variations, the ratio between peak-to-peak difference of
the real measured output (Msd_sig.) and the reference
output (Ref_sig.) is calculated, and this value is propor-
tional to the current gas concentration. Actually, a rela-
tion curve between this ratio and the gas concentration has
already established when performing calibration of the
system at a discrete concentration step, and has been stored
Figure 3. The instrument module
Figure 4. GUI for CO2 detection written in MATLAB
in the EEPROM inside the MCU. The measured CO2 gas
concentration can then be calculated using the ordinary
least-square interpolation method in between two
neighboring discrete concentration values designed in
calibration period.
As a human machine interface, the calculated real
concentration is also shown on the LCD.
In the paper, we introduced the method of concentration
detection for human exhaled CO2 gas, described the
functional module (the detection module and the instru-
ment module), and provided the GUI for CO2 detection
written in MATLAB.
The experiment result shows that it is a practical sys-
tem for medical CO2 concentration detection whose
measurement range is from 0 to 3000 ppm (part per mil-
lion; 1000ppm =0.1%) with ±5% of reading accuracy as
desired, and it will give benefits for the development of
our medical care in the future, as expected.
However, there are still remaining challenges ahead of
us before real practical use. Firstly, the characteristics of
the system need to be verified for relatively long time.
Secondly, a face mark for the patients receiving supple-
mental oxygen should be considered in the mechanical
design. To provide additional functions, such as remote
control of the system are also needed to be considered in
the future for specific environments.
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