Response of Explants of Calli Rice (Oryza sativa L.) Japonica cv. “Ilmi” to Gene Transformation Using Agrobacterium tumefaciens-Mediated

Fika Ayu Safitri, Mohammad Ubaidillah, Miswar  , Kyung-Min Kim

Agrotechnology Study Program, Faculty of Agriculture, Jember University, Jember, Indonesia.
Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, Daegu, South Korea.

DOI: 10.4236/ajps.2013.44102                

Abstract

The AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor was introduced into Japonica cultivars of rice (“Ilmi”) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice calluses. Introduction of the AtBI-1 gene was also confirmed by PCR using AtBI-1 specific oligonucleotide primers in regenerated plants. Stable integration and expression of the AtbI-1 gene in plants were confirmed by GFP analysis.

Keywords

AtBI-1; Agrobacterium-Mediated; GFP; Transformation; Rice

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	K. F. Kiple and K. C. Ornelas, “The Cambrigde World: History of Food,” Cambridge University Press, Cambridge, 2000.
[2]	M. Kubo and M. M. Purevdorj, “The Future of Rice Production and Consumption,” Journal of Food Distribution Research, Vol. 35, No. 1, 2004, pp. 128-142.
[3]	S. J. Lim, H. G. Hwang, S. J. Yang, B. G. Oh, U. S. Yoe, N. B. Park, H. Y. Kim, G. H. Yi, B. Y. Jun, S. C. Kim, S. K. Lee, Y. J. Oh and G. S. Chung, “High Eating Quality and Medium-Late Maturing Rice Variety with Resistance to Diseases and Lodging ‘Ilmi’,” Korean Journal of Breeding Science, Vol. 28, No. 4, 1996, pp. 481-481.
[4]	Y. Duan, C. Zhai, H. Li, J. Li, W. Mei, H. Gui, D. Ni, F. Song, L. Li, W. Zhang and J. Yang, “An Efficient and High through Protocol for Agrobacterium Mediated Transformation Based on Phosphomannose Isomerase Positive selection in Japonica Rice (Oryza sative L.),” Plant Cell Reports, Vol. 31, No. 9, 2012, pp. 1611-1624. doi:10.1007/s00299-012-1275-3
[5]	N. Wanatabe and E. Lam, “Bax Inhibitor-1, Conversed Call Death Suppressor, Is a Key Molecular Switch Downstream from a Variety of Biotic and Abiotic Stress Signals in Plants,” International Journal of Molecular Sciences, Vol. 10, No. 7, 2009, pp. 3149-3167. doi:10.3390/ijms10073149
[6]	N. Wanatabe and E. Lam, “Arabidopsis Bax Inhibitor-1 Arheostat for ER Stress-Induced Programmed Cell Death,” Plant Signaling & Behaviour, Vol. 3, No. 8, 2008, pp. 564-566. doi:10.4161/psb.3.8.5709
[7]	M. Kawai, L. Pan, J. C. Reed and H. Uchimiya, “Evolutionally Conserved Plant Homologue of the Bax Inhibitor-1 (BI-1) Gene Capable of Suppressing Bax-Induced Cell Death in Yeast,” FEBS Letters, Vol. 464, No. 3, 1999, pp. 143-147. doi:10.1016/S0014-5793(99)01695-6
[8]	P. D. Kasi, Sumaryono, “Development of Embryogenic Callus Sago (Metroxylon sagu Rottb.) on Three in Vitro Culture System,” Tower Estates, Vol. 76, No. 1, 2008, pp. 1-10.
[9]	W. T. Peng, Y. W. Lee and E. N. Nester, “The Phenolic Recognition Profiles of the Agrobacterium tumefaciens Vir Aprotein Are Broadened by a High Level of the Sugar Binding Protein ChvE,” Journal of Biotechnology, Vol. 190, No. 21, 1998, pp. 5632-5638.
[10]	I. Nijmona and T. Lamparter, “Temperature Effects on Agrobacterium Phytochrome Agp1,” PLoS ONE, Vol. 6, No. 10, 2011, p. e25977. doi:10.1371/journal.pone.0025977
[11]	I. Oberpichler, R. Rosen, A. Rasouly, M. Vugman, E. Z. Ron and T. Lamparter, “Light Affects Motolity and Infectivity of Agrobacterium tumefaciens,” Environmental Microbiology, Vol. 10, No. 8, 2008, pp. 2020-2029. doi:10.1111/j.1462-2920.2008.01618.x