Author(s)

Saúl Flores-Medina^1,2,3*, Carmen S. García-Romero⁴, Diana M. Soriano-Becerril⁴, Francisco J. Díaz-García⁵, Silvia Giono-Cerezo⁶, Graciela Castro-Escarpulli⁶

¹Departamento Editorial, Instituto Nacional de Perinatología, Ciudad de México, México.
²CECyT No. 15 “DAE”, Instituto Politécnico Nacional, Ciudad de México, México.
³Programa de Biología y Biotecnología Molecular, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México.
⁴Departamento de Infectología, Instituto Nacional de Perinatología, Ciudad de México, México.
⁵Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México.
⁶Laboratorio de Bacteriología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México.

Aims: To assess the frequency and the main HPV genotypes circulating among a group of women attending at a third level Hospital in Mexico City. Methods: A cross-sectional and descriptive study was performed in a group of 143 female outpatients of the Gynecology and Obstetrics Service at the National Institute of Perinatology of Mexico. Cervical swabs were taken from participants and subjected to simultaneous detection/genotyping of HPV by Linear Array Genotyping Test (Roche Molecular Systems). Mann-Whitney U, median and/or Square Chi tests were used to compare socio-demographical features between HPV-infected and uninfected women. Results: A total of 66 women (46.2%) had HPV infection. Overall, 112 genotypes were detected either as single infections (45.5%) or multiple genotype infections (54.5%). The cumulated frequency of multiple infections with high-/low- and high-/high-risk HPV genotypes was 63.9 %. The most frequent high-risk genotypes were HPV52 HPV58 and HPV51, whereas the most frequent low-risk genotypes were HPV6, HPV53 and HPV84. Infected women were significantly younger and have less stable partner relationships than uninfected women (p < 0.05). Conclusion: A relevant frequency of mixed infections with high- and low-risk HPV genotypes, other than those considered most prevalent worldwide, was observed. Most circulating high-risk genotypes among the women of this study are not covered by commercial vaccine formulations.

Human Papillomavirus, High-Risk Genotypes, HPV Co-Infections, Linear Array Genotyping

Flores-Medina, S. , García-Romero, C. , Soriano-Becerril, D. , Díaz-García, F. , Giono-Cerezo, S. and Castro-Escarpulli, G. (2016) Relevant Frequency of Multiple Infections with High- and Low-Risk HPV Genotypes among Mexican Women Attending a Tertiary Care Hospital. Open Journal of Obstetrics and Gynecology, 6, 424-432. doi: 10.4236/ojog.2016.67056.