Engineering Antibody Fragment with the Quantum Dot in Cancer Cell Imaging and Diagnosis - Engineering

ENG > Vol.4 No.10, October 2012

Engineering Antibody Fragment with the Quantum Dot in Cancer Cell Imaging and Diagnosis ()

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Download as PDF (Size: 280KB) PP. 126-128

DOI: 10.4236/eng.2012.410B033 3,540 Downloads 4,907 Views Citations

Author(s)

Lizhi Liu, Weiming Xu

Affiliation(s)

Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Firth Court, Sheffield, UK.

ABSTRACT

The conjugates of monoclonal antibodies and nanoparticles, including quantum-dot(QD), offer significant advantages over conventional fluorescent probes to image and study biological processes. The extend stability, intense fluorescence and low toxicity of QDs are well suited for biological applications. In the present study, we used QD-conjugated anti-glucose-regulated protein 78(GRP78) antibody to examine the gene expression in prostate cancer cells under the unfolded protein response (UPR). QDs have got unique, simple and fast properties over current diagnostic techniques such as peroxidase-based immunohistochemical staining procedures, therefore the nanocarrier-conjugated antibody fragment has potential to become a new therapeutic tool for cancer diagnosis and treatment.

KEYWORDS

Quantum Dot; Antibody Fragment; Prostate Cancer; GRP78; scFv; UPR; Immunohistochemistry

Share and Cite:

L. Liu and W. Xu, "Engineering Antibody Fragment with the Quantum Dot in Cancer Cell Imaging and Diagnosis," Engineering, Vol. 4 No. 10B, 2012, pp. 126-128. doi: 10.4236/eng.2012.410B033.

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