Author(s)

Govindhan Varadharajan, Lakshmanan Rajendran

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A closed form of an analytical expression of concentration in the single-enzyme, single-substrate system for the full range of enzyme activities has been derived. The time dependent analytical solution for substrate, enzyme-substrate complex and product concentrations are presented by solving system of non-linear differential equation. We employ He’s Homotopy perturbation method to solve the coupled non-linear differential equations containing a non-linear term related to basic enzymatic reaction. The time dependent simple analytical expressions for substrate, enzyme-substrate and free enzyme concentrations have been derived in terms of dimensionless reaction diffusion parameters ε, λ₁, λ₂ and λ₃ using perturbation method. The numerical solution of the problem is also reported using SCILAB software program. The analytical results are compared with our numerical results. An excellent agreement with simulation data is noted. The obtained results are valid for the whole solution domain.

Non-Linear Reaction Equations, Enzyme Inactivation, Homotopy Perturbation Method, Time Dependent Analytical Solution

G. Varadharajan and L. Rajendran, "Analytical Solutions of System of Non-Linear Differential Equations in the Single-Enzyme, Single-Substrate Reaction with Non-Mechanism-Based Enzyme Inactivation," Applied Mathematics, Vol. 2 No. 9, 2011, pp. 1140-1147. doi: 10.4236/am.2011.29158.