A Study of Correlation of Serum Chromium Level with Glycosylated Haemoglobin (HbA1c), Total Cholesterol and Triglycerides, among Type 2 Diabetes Patients ()
Abstract
Background: Diabetes mellitus is a common disease and it is a major cause of morbidity; several studies indicate that diabetes is a likely under reported cause of death. Chromium’s is important trace element to control diabetes mellitus and metabolism of carbohydrate, lipid and protein. Objective: The purpose of this study was to understand the relationship between serum chromium, with HbA1c, Total cholesterol and Triglycerides among type 2 diabetes patients among diabetic patients. Methodology: This is cross-sectional study done in Jabber Abu Ezz Centre for treatment and care of diabetics in Khartoum—Sudan. Four hundred subjects were enrolled in this study; one hundred subjects were normal healthy as control group, and three hundred subjects diabetic patient type 2 as test group; demographic and biochemical data were collected; serum chromium, Glycosylated Haemoglobin (HbA1c), Total cholesterol Triglycerides, were determined by using NYCOCARD READER II, spectrophotometer (Biosystem 310) and spectrophotometer 210-VGP. Result: In this study there is significant parameters level means of FBS HbA1c, Total Cholesterol, Triglycerides and Chromium of the test groups when compared with healthy control groups subjects (P = 0.001, 0.018, 0.01, 0.011, 0.004), respectively. Significant negative correlation is between FBS, HbA1c, Total Cholesterol, Triglycerides and Chromium (r = ?0.555, P value = 0.003), (r = ?0.668, P value = 0.002), (r = ?0.335, P value = 0.004) and (r = ?0.774, P value = 0.002) respectively. Conclusion: There was significant correlation between serum Chromium level with fasting blood sugaer, Glycosylated Haemoglobin (HbA1c), Total cholesterol and Triglycerides among type 2 diabetes patients.
1. Introduction
Diabetes mellitus (DM) is a group of metabolic diseases characterized by increased blood glucose level resulting from defects in insulin secretion, insulin action, or both [1] . Dyslipidemia is a major risk factor for coronary heart disease. Type 2 diabetes is associated with a cluster of interrelated plasma lipid and lipoprotein abnormalities [2] .
The metabolism of several minerals has been reported to alter in diabetes mellitus and these elements might have specific role in the pathogenesis and progress of the disease. Among these the trace elements-chromium is important for the growth and biological functions [3] . Type 2 diabetes is the commonest form of diabetes and associated with multiple metabolic derangements that result in the excessive production of reactive oxygen species (ROS) and oxidative stress [4] . In diabetes, there are more mechanisms that induce oxidative stress than in normal individuals; such as, glucose auto-oxidation, non-enzymatic glycation of protein, and polyol pathway. These pathways enhance generation of reactive oxygen species (ROS) leading to the tissue damage and causing several complex syndromes in diabetic patients such as cataracts, renal dysfunction, nerve damage, and atherosclerosis. Especially, atherosclerosis leading to the coronary heart disease (CHD) is the major cause of death among diabetics [5] . Trace elements participate in production of reactive oxygen species (ROS), which contribute to oxidative stress. Oxidative stress contributes to the pathogenesis of many diseases including DM. Chromium an essential trace element as well as a vital antioxidant, plays an important role in glucose and lipid metabolism by improving glucose intolerance and lowering elevated lipids. Chromium deficiency affects the maintenance of normal glucose tolerance and healthy lipid profiles [6] . Thus, the aim of our study was to find out the association of the chromium with lipid profile and glycemic control in the patients of type 2 diabetes mellitus.
2. Materials and Methods
The study was a descriptive, case control hospital based study. 100 healthy subject were control group with mean FBS 5.3 m・mol/L, they were 59 males and 41 females. The age ranged from 27 to 66 years old. The mean age average was 46.67 years. Type 2 diabetic patients were 300 collected by simple random method, the ages ranged from 27 to 78 years old. The mean age average was 46.62 years. Data were collected using questionnaire. All samples were in a state of fasting for 12 hours, blood specimen (7 ML) was collected and we excluding all over fasting patients, samples kept in a test tube at room temperature then clotted blood sample was centrifuged at 4000 r.p.m and separated, then quickly stored at −20˚C till used during the period between March 2014 until January 2017. The study has been approved by the local ethics committee of Medical Director of all hospital. All participants in the study were given their written informed consent considering the aims of the study and sample and clinical information’s were used anonymously.
HbA1c assay was done by method based on boronate affinity chromatography using NYCOCARD READER II. Fasting Blood Glucose, Total Cholesterol and Triglycerides was measured by enzymatic method by using spectrophotometer (Biosystem 310) Biosystem Kit Company. Chromium serum level was measured using atomic absorption spectrophotometer 210-VGP Electron of the atom promoted to higher orbital’s (excited state) for a short period of time by absorbing a defined quantity of energy. The amount of energy was specific to a particular electron transition in a particular element (chromium). The radiation measured by using detector and the absorbance was converted to analytic concentration. Brief according to the manufacture’s protocol, serum chromium was diluted 1:9 with deionized, then diluted serum aspirated and absorbance was measured at wave length 213.9 nm.
3. Results
Table 1 shows in this study there is significant parameters level means of FBS HbA1c, Total Cholesterol, Triglycerides and Chromium of the test groups when compared with healthy control groups subjects (P = 0.001, 0.018, 0.01, 0.011, 0.004), respectively. Significant negative correlation between FBS, HbA1c, Total Cholesterol, Triglycerides and Chromium (r = −0.555, P value = 0.003), (r =
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Table 1. Comparison of the means of Blood Parameters between diabetics and none diabetics.
*Significant differences in all blood parameters between control and test group (P value < 0.05).
−0. 668, P value = 0.002), (r = −0.335, P value = 0.004) and (r = −0.774, P value = 0.002) 0.001, P = 0.012) (Figures 1-4) respectively.
4. Discussion
The present study showed that a significant elevation of the means of the serum levels of total cholesterol of the test group when compared with the control group, these findings agree with the result of P, Annapurna et al. [7] , who found significant raised in the means of the plasma levels of total. In our study the diabetic patients have a significant reduction in the means of their serum levels of
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Figure 1. Scatter plot shows the relationship between serum chromium and serum FBS of the test group (N = 300) (r= −0.555, P value = 0.003).
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Figure 2. Scatter plot shows the relationship between HbA1c% and serum chromium of the test group (N = 300) (r = −0.668, P value = 0.002).
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Figure 3. Scatter plot shows the relationship between serum chromium and serum total cholesterol of the test group (N = 300) (r = −0.335, P value = 0.004).
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Figure 4. Scatter plot shows the relationship between serum chromium and serum triglycerides cholesterol of the test group (N = 300) (r = −0.774, P value = 0.002).
cholesterol when compared to the control group, this result agrees with that reported by Rosmee and K. Shyamal [8] , who found the mean of the serum levels of HDLc was significantly lower in diabetic obese patients than in the control subjects. A study done by Wexler et al. [9] obtained a result that was not in concordance with our result in which the lipid profile were higher in diabetic patients. In our study we found the diabetic patients have a significant elevation in the means of the serum levels of LDL cholesterol when compared to the control group, this result agrees with that reported by M, G Bhutto [10] in a study done in India, who found the mean of the serum levels of LDLc was significantly elevation in diabetic patients than in the control subjects. The current study shows a significant elevation of the mean of the serum levels of triglycerides among the diabetic patients when compared to the control group, this agrees with a study done by Sarkar Chandra Bidan [11] , who found the mean of the serum levels of triglycerides of the test group was significantly raised when compared with the control group. In our study the diabetic patients have a significant reduction in the means of the serum levels of chromium when compared to the control group, this result agrees with that reported by Rajendran Kannan et al. [12] , who found the mean of the serum levels of chromium was significantly lower in diabetic patients than in the control subjects, this is in coordination with the findings of Morris et al. [13] , Ekmekcioglu et al. [14] , and Kazi et al. [15] that indicated lower blood levels of chromium in diabetics than in nondiabetics. The explanation of this result can be obtained from other studies which showed that the rates of absorption and excretion of chromium in diabetics are more than in nondiabetics (Anderson et al. also SHRABANI M et al. [16] [17] ; Hamad et al. [18] ). As a result of this, supplemental Cr was tried and found to have beneficial effects on people with glucose intolerance, diabetes, obesity, and neuropathy had experiments that showed reduction in blood glucose levels as a response to chromium supplementation [16] [18] . In our study we observed a significant Negative correlation between the serum levels of total cholesterol and the serum chromium, this result agrees with that reported by A, I. Ahmed, & M, M. Helal et al. [19] , in a study done in Egypt, who found a significant Negative correlation between the serum chromium and the serum levels of the total cholesterol. In our study shows negative correlation between the serum levels of triglycerides cholesterol and the serum chromium, this agrees with that reported by Ahmed, & M, M. Helal et al. [19] , in a study done in Egypt, who found negative correlation between the serum triglycerides cholesterol and the serum chromium.
5. Conclusion
There was significant correlation between serum Chromium level with fasting blood sugaer, Glycosylated Haemoglobin (HbA1c), Total Cholesterol and Triglycerides among type 2 diabetes patients. Deficient chromium level, as their vital role in lipids metabolism and insulin function chromium supplementation are essential in the control of glycemic metabolism and lipid profile in DM2.
Acknowledgements
We are grateful to them all for participating in our study and special thanks to the staff of Jabber Abu Ezz Centre for treatment and care of diabetics in Khartoum Sudan.