Share This Article:

Serum Resistin Level and Polymorphonuclear Leukocytes Dysfunctions in Children on Regular Hemodialysis

Abstract Full-Text HTML XML Download Download as PDF (Size:614KB) PP. 423-430
DOI: 10.4236/ijcm.2015.66055    2,761 Downloads   3,095 Views   Citations

ABSTRACT

Resistin is a secretory adipocytoine, which is expressed mainly in humans by inflammatory cells especially macrophages. Resistin serum levels are elevated in end-stage renal diseases of people having an increased risk of infections as a result of impaired polymorphonuclear leukocytes (PMNLs) functions. Objectives: To evaluate neutrophil functions (phagocytosis and oxidative burst) in children with end-stage renal disease (ESRD) on regular hemodialysis and to shed light on the contribution of resistin on neutrophil functions. Patients and Methods: The study included 40 children with ESRD on regular hemodialysis. Their ages ranged from 6 to 12 years, and they were selected from children attending the pediatric hemodialysis unit of AL-Zahraa Hospital, Al-Azher University during the period from October 2012 to December 2013. Another group of 40 apparently healthy children with matched age and sex with the patient group served as a control. Serum resistin, phagocytic index and nitro blue tetrazolium test (NBT%) were assessed in both groups. Results: There was a statistically more significant increase in resistin serum levels in cases than in controls; it was (3.25 ± 0.86 ng/ml) and (0.25 ± 0.16 ng/ml) respectively (P < 0.01). On the other hand there was a statistically more significant decrease in neutrophil phagocytic index in cases than in controls; it was (2.57 ± 1.34) and (3.55 ± 0.74) respectively (P < 0.01). Also it showed a statistically more significant decrease in NBT% in cases than in controls; it was (47.98 ± 16.38%) and (61.45 ± 13.17%) respectively (P < 0.01). We found negative correlation between resistin serum level with phagocytic index and NBT%, while we found positive correlation between resistin serum level and hemodialysis duration. Conclusion: High resistin serum level in children with ESRD decreases phagocyte function and oxidative burst of PMNLs, and this is enhanced by the longer duration of hemodialysis.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Zein, M. , Abdel-Salam, M. , Abdel-Aziz, I. and Mohamed, N. (2015) Serum Resistin Level and Polymorphonuclear Leukocytes Dysfunctions in Children on Regular Hemodialysis. International Journal of Clinical Medicine, 6, 423-430. doi: 10.4236/ijcm.2015.66055.

References

[1] Mayadas, T.N., Cellere, X. and Towell, C.A. (2014) The Multifaceted Functions of Neutrophils. Annual Review of Pathology: Mechanism of Disease, 9, 181-218.
http://dx.doi.org/10.1146/annurev-pathol-020712-164023
[2] McDonald, B., Urrutia, R., Jenne, C.N. and Kubes, P. (2012) Intravascular Neutrophil Extracellular Traps Capture Bacteria from the Bloodstream during Sepsis. Cell Host Microbe, 12, 324-333.
http://dx.doi.org/10.1016/j.chom.2012.06.011
[3] Vaziri, N.D., Pahl, M.V., Crum, A. and Noris, K. (2012) Effect of Uremia on Structure and Function of Immune System. Journal of Renal Nutrition, 22, 149-156.
http://dx.doi.org/10.1053/j.jrn.2011.10.020
[4] Mocsai, A. (2013) Diverse Novel Functions of Neutrophils in Immunity, Inflammation and Beyond. Journal of Excremental Medicine, 210, 1283-1299.
http://dx.doi.org/10.1084/jem.20122220
[5] Teta, D. (2012) Adipokines as Uremic Toxins. Journal of Renal Nutrition, 22, 81-85.
http://dx.doi.org/10.1053/j.jrn.2011.10.029
[6] Korpela, L.S., Lehtimaki, L., Vuolteenaho, K., Nieminen, R., Saarelainen, H.K.S. and Moilanen, E. (2011) Adipokine Resistin Predicts Anti-Inflammatory Effect of Glucocorticoids in Asthma. Journal of Inflammation, 8, 12.
http://dx.doi.org/10.1186/1476-9255-8-12
[7] Patel, S.P. and Raju, P.A. (2013) Resistin in Serum and Gingival Cervical Fluid as a Marker of Periodontal Inflammation and Its Correlation with Single-Nucleotide Polymorphism in Human Resistin Gene at -420. Dental College and Research Institute, Bangalore, 192-197.
[8] Cohen, G., Ilic, D., Raupachova, J. and Ho, W.H. (2008) Resistin Inhibits Essential Functions of Polymorphonuclear Leukocyte. Journal of Immunology, 181, 3761s-3768s.
http://dx.doi.org/10.4049/jimmunol.181.6.3761
[9] Muniz-Junqueira, M.I,. Pecanha, L.M., de Silva-Filho, V.L., de Almeida, Cardoso, M.C. and Tosta, C.E. (2003) Novel Micotechnique for Assessment of Postnatal Maturation of the Phagocytic Function of Neutrophil and Monocytes. Clinical and Diagnostic Laboratory Immunology, 10, 1096-1102.
[10] Carneir, O.V.M., Bezerra, A.C., Machado, M. do C., Guimaraes, M.D. and Muniz-Junqueira, M.I. (2012) Decreased Phagocytic Function in Neutrophils and Monocytes from Peripheral Blood in Periodontal Disease. Journal of Applied Oral Science, 20, 5.
http://dx.doi.org/10.1590/s1678-77572012000500002
[11] Almehed, K., d’Elia, H.F., Bokarewa, M. and Carlsten, H. (2008) Role of Resistin as a Marker of Inflammation in Systemic Lupus Erythematosus. Arthritis Research & Therapy, 10, R15.
http://dx.doi.org/10.1186/ar2366
[12] Kader, N.A., Ali El-Din, F.A., Khatab, E.A.A. and Elabd, N.E. (2010) Does Plasma Resistin Level Have a Role in Predicting Inflammatory Bowel Disease Activity? Indian Journal of Gastroenterology, 29, 126-127.
http://dx.doi.org/10.1007/s12664-010-0008-1
[13] Nehus, E., Furth, S., Warady, B. and Mitsnefes, M. (2012) Correlates of Resistin in Children with Chronic Kidney Disease: The Chronic Kidney Disease in Children Cohort. The Journal of Pediatrics, 161, 276-280.
http://dx.doi.org/10.1016/j.jpeds.2012.01.055
[14] Zaza, G., Granata, S., Rascio, F., Pontrelli, P., Dell, M., Cox, S.H., Pertosa, G., Grandaliano, G. and Lupo, A. (2013) A Specific Immune Transcriptomic Profile Discriminates Chronic Kidney Disease Patients in Predialysis from Hemodialyzed Patients. BMC Medical Genomics, 6, 17.
http://dx.doi.org/10.1186/1755-8794-6-17
[15] Guo, H., Liu, J., Collins, A.J. and Foley, R.N. (2008) Pneumonia in Incident Dialysis Patients—The United States Renal Data System. Nephrology Dialysis Transplantation, 23, 680-686.
http://dx.doi.org/10.1093/ndt/gfm474
[16] Sardenberg, C., Suassuna, P., Andreoli, M.C., Watanabe, R., Dalboni, M.A., Manfredi, S.R., et al. (2006) Effects of Uraemia and Dialysis Modality on Polymorphonuclear Cell Apoptosis and Function. Nephrology Dialysis Transplantation, 21, 160-165.
http://dx.doi.org/10.1093/ndt/gfi095
[17] Gómez-Ochoa, P., Sabateb, D., Homedesb, J. and Ferrerc, L. (2012) Use of the Nitroblue Tetrazolium Reduction Test for the Evaluation of Domperidone Effects on the Neutrophilic Function of Healthy Dogs. Veterinary Immunology and Immunopathology, 146, 97-99.
http://dx.doi.org/10.1016/j.vetimm.2012.01.018
[18] Libetta, C., Sepe, V., Esposito, P. and Galli, F. (2011) Oxidative Stress and Inflammation: Implications in Uremia and Hemodialysis. Clinical Biochemistry, 44, 1189-1198.
http://dx.doi.org/10.1016/j.clinbiochem.2011.06.988
[19] Ghobrial, E.E., Mahfouz, N.N., Fathy, G.A., El Wakkad, A.A. and Sebaii, H.M. (2013) Oxidative Stress in Egyptian Heamodialysis Children. Iranian Journal of Kidney Diseases, 7, 485-491.
[20] Nusken, K.D., Kratzsch, J., Wienholz, V., Stohr, W., Rascher, W. and Dotsch, J. (2006) Circulating Resistin Concentrations in Children Depend on Renal Function. Nephrology Dialysis Transplantation, 21, 107-112.
http://dx.doi.org/10.1093/ndt/gfi084
[21] Mehdi, W.A. (2011) Determination of Resistin and Several Antioxidants in Sera of Patients with Chronic Renal Failure. Australian Journal of Basic and Applied Sciences, 5, 196-203.
[22] Abraham, G., Sundaram, V., Sundaram, V., Mathew, M., Leslie, N. and Sathiah, V. (2009) C-Reactive Protein, a Valuable Predictive Marker in Chronic Kidney Disease. Saudi Journal of Kidney Diseases and Transplantation, 20, 811-815.
[23] Shroff, R. (2009) Monitoring Cardiovascular Risk Factors in Children on Dialysis. Peritoneal Dialysis International, 29, S173-S175.
[24] Gheissari, A., Kelishadi, R., Roomizadeh, P., Abedini, A., Haghjooy-Javanmard, S., Abtahi, S.H., et al. (2013) Chronic Kidney Disease Stages 3-5 in Iranian Children: Need for a School-Based Screening Strategy: The CASPIAN-III Study. International Journal of Preventive Medicine, 4, 95-101.
[25] Ohtake, T., Ishioka, K., Honda, K., Oka, T., Maesato, K., Mano, T., Ikee, R., Moriya, H., Hidaka, S. and Kobayashi, S. (2010) Impact of Coronary Artery Calcification in Hemodialysis Patients and Association with Prognosis. Hemodialysis International, 14, 218-225.
http://dx.doi.org/10.1111/j.1542-4758.2009.00423.x

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.