Share This Article:

Preliminary Clinical Study Using a Novel Wound Dressing Composed of Hyaluronic Acid and Collagen Containing EGF

DOI: 10.4236/ojrm.2015.41002    3,484 Downloads   4,202 Views   Citations

ABSTRACT

This clinical trial aimed to evaluate the efficacy and safety of a novel wound dressing composed of hyaluronic acid (HA) and collagen (Col) containing epidermal growth factor (EGF), referred to as EGF-wound dressing. EGF-wound dressing was prepared by freeze-drying a mixed aqueous solution of high-molecular-weight HA, low-molecular-weight HA and heat-denatured Col containing EGF. EGF-wound dressing was applied to skin defects, such as intractable skin ulcers, burn ulcers, traumatic skin defects and skin donor-site wounds. The dressing was changed twice a week for a period of 6 weeks or longer, if necessary. The primary endpoints were size of wound area, formation of granulation tissue, extent of epithelialization, infection control and macroscopic appearance. Effectiveness, safety and overall clinical evaluation were scored by plastic surgeons, as authorized by the Japanese Society of Plastic and Reconstructive Surgery. This study was registered with the University Hospital Medical Information Network (UMIN0000005264). Healthy granulation tissue and rapid epithelialization were observed for a given period after application of EGF-wound dressing onto the wounds. Most cases were assessed as having achieved good or excellent results. This clinical study demonstrated that EGF-wound dressing was beneficial in the treatment of various skin defects.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Yu, A. , Takeda, A. , Kumazawa, K. , Miyoshi, H. , Kuroyanagi, M. , Yoshitake, T. , Uchinuma, E. , Suzuki, R. and Kuroyanagi, Y. (2015) Preliminary Clinical Study Using a Novel Wound Dressing Composed of Hyaluronic Acid and Collagen Containing EGF. Open Journal of Regenerative Medicine, 4, 6-13. doi: 10.4236/ojrm.2015.41002.

References

[1] Singer, A.J. and Clark, R.A. (1999) Cutaneous Wound Healing. New England Journal of Medicine, 341, 738-746.
http://dx.doi.org/10.1056/NEJM199909023411006
[2] Moulin, V. (1995) Growth Factors in Skin Wound Healing. European Journal of Cell Biology, 68, 1-7.
[3] Kondo, S. and Kuroyanagi, Y. (2012) Development of a Wound Dressing Composed of Hyaluronic Acid and Collagen Sponge with Epidermal Growth Factor. Journal of Biomaterials Science, Polymer Edition, 23, 629-643.
http://dx.doi.org/10.1163/092050611X555687
[4] Yu, A., Matsuda, Y., Takeda, A., Uchinuma, E. and Kuroyanagi, Y. (2012) Effect of EGF and bFGF on Fibroblast Proliferation and Angiogenic Cytokine Production from Cultured Dermal Substitutes. Journal of Biomaterials Science, Polymer Edition, 23, 1315-1324.
[5] Kondo, S., Niiyama, H., Yu, A. and Kuroyanagi, Y. (2012) Evaluation of a Wound Dressing Composed of Hyaluronic Acid and Collagen Sponge Containing Epidermal Growth Factor in Diabetic Mice. Journal of Biomaterials Science, Polymer Edition, 23, 1729-1740.
http://dx.doi.org/10.1163/092050611X555687
[6] Yu, A., Niiyama, H., Kondo, S., Yamamoto, A., Suzuki, R. and Kuroyanagi, Y. (2013) Wound Dressing Composed of Hyaluronic Acid and Collagen Containing EGF or bFGF: Comparative Culture Study. Journal of Biomaterials Science, Polymer Edition, 24, 1015-1026.
http://dx.doi.org/10.1080/09205063.2012.731375
[7] Niiyama, H. and Kuroyanagi, Y. (2014) Development of Novel Wound Dressing Composed of Hyaluronic Acid and Collagen Sponge Containing Epidermal Growth Factor and Vitamin C Derivative. Journal of Artificial Organs, 17, 81-87.
http://dx.doi.org/10.1007/s10047-013-0737-x
[8] Chen, W.Y. and Abatangelo, G. (1999) Functions of Hyaluronan in Wound Repair. Wound Repair and Regeneration, 7, 79-89.
http://dx.doi.org/10.1046/j.1524-475X.1999.00079.x
[9] Laurent, T.C. and Fraser, J.R. (1992) Hyaluronan. FASEB Journal, 6, 2397-2404.
[10] West, D.C., Hampson, I.N., Arnold, F. and Kumar, S. (1985) Angiogenesis Induced by Degradation Products of Hyaluronic Acid. Science, 228, 1324-1326.
http://dx.doi.org/10.1126/science.2408340
[11] Sattar, A., Rooney, P., Kumar, S., Pye, D., West, D.C., Scott, I. and Ledger, P. (1994) Application of Angiogenic Oligosaccharides of Hyaluronan Increases Blood Vessel Numbers in Rat Skin. Journal of Investigative Dermatology, 103, 576-579.
http://dx.doi.org/10.1111/1523-1747.ep12396880
[12] Lees, V.C., Fan, T.P. and West, D.C. (1995) Angiogenesis in a Delayed Revascularization Model Is Accelerated by Angiogenic Oligosaccharides of Hyaluronan. Laboratory Investigation, 73, 259-266.
[13] Postlethwaite, A.E., Seyer, J.M. and Kang, A.H. (1978) Chemotactic Attraction of Human Fibroblasts to Type I, II, and III Collagens and Collagen-Derived Peptides. Proceedings of the National Academy of Sciences of the United States of America, 75, 871-875.
http://dx.doi.org/10.1073/pnas.75.2.871

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.