Share This Article:

First Characterization of Escherichia coli Strains Isolated from Wildlife Griffon Vulture (Gyps fulvus) in the Southeast of Spain

Full-Text HTML XML Download Download as PDF (Size:2509KB) PP. 329-333
DOI: 10.4236/ojvm.2014.412040    2,765 Downloads   3,210 Views   Citations

ABSTRACT

The aim of the present study was to characterize a collection of Escherichia coli strains isolated from asymptomatic griffon vulture (Gyps fulvus) during a reintroduction program in the southeast of Spain, in order to establish if griffon vulture could play a role in the spread of resistant or potentially pathogenic E. coli strains. For this purpose, 14 E. coli strains obtained from 10 griffon vulture were studied to establish their serotypes, phylogroups, virulencegene profiles and antimicrobial resistances. High heterogeneity was observed within the 14 strains isolated which belonged to three phylogroups (A, B1 and D), 8 serogroups (O2, O21, O29, O60, 073, O78, O103 and O141) and 13 different serotypes. Out of 34 genes screened, we have detected eight virulence genes that are typical of extraintestinal pathogenic E. coli (ExPEC) (fimH, fimAvMT78, iroN, iucD, cvaC, iss, traT and tsh); however, none of the studied strains showed the ExPEC status. The 14 strains were also analyzed for the production of extended-spectrum beta-lactamases (ESBLs) and for antimicrobial resistances. None of the 14 strains were ESBL-producing E. coli, but high resistance-prevalences to ampicillin and cotrimoxazole were detected. To our knowledge, this is the first characterization of E. coli strains isolated from griffon vulture and although they did not show high virulencegene scores, they showed cotrimoxazole resistance.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Mora, A. , Ortega, N. , Garcia, E. , Viso, S. , Candela, M. , Dahbi, G. , Cuello, F. and Caro, M. (2014) First Characterization of Escherichia coli Strains Isolated from Wildlife Griffon Vulture (Gyps fulvus) in the Southeast of Spain. Open Journal of Veterinary Medicine, 4, 329-333. doi: 10.4236/ojvm.2014.412040.

References

[1] Loria, G.R., Ferrantelli, E., Giardina, G., Li Vecchi, L., Sparacino, L., Oliveri, F., McAuliffe, L. and Nicholas, R.A. (2008) Isolation and Characterization of Unusual Mycoplasma spp. from Captive Eurasian Griffon (Gyps fulvus) in Sicily. Journal of Wildlife Diseases, 44, 159-163.
http://dx.doi.org/10.7589/0090-3558-44.1.159
[2] Darwich, L., Cabezón, O., Echeverria, I., Pabón, M., Marco, I., Molina-López, R., Alarcia-Alejos, O., López-Gatius, F., Lavín, S. and Almería, S. (2012) Presence of Toxoplasma gondii and Neospora caninum DNA in the Brain of Wild Birds. Veterinary Parasitology, 183, 377-381.
http://dx.doi.org/10.1016/j.vetpar.2011.07.024
[3] Mora, A., Herrrera, A., López, C., Dahbi, G., Mamani, R., Pita, J.M., Alonso, M.P., Llovo, J., Bernárdez, M.I., Blanco, J.E., Blanco, M. and Blanco, J. (2011) Characteristics of the Shiga-Toxin-Producing Enteroaggregative Escherichia coli O104:H4 German Outbreak Strain and of STEC Strains Isolated in Spain. International Microbiology, 14, 121- 141.
[4] Blanco, J.E., Blanco, M., Mora, A., Jansen, W.H., García, V., Vázquez, M.L. and Blanco, J. (1998) Serotypes of Escherichia coli Isolated from Septicaemic Chickens in Galicia (Northwest Spain). Veterinary Microbiology, 61, 229- 235.
http://dx.doi.org/10.1016/S0378-1135(98)00182-5
[5] Mora, A., Viso, S., López, C., Alonso, M.P., García-Garrote, F., Dabhi, G., Mamani, R., Herrera, A., Marzoa, J., Blanco, M., Blanco, J.E., Moulin-Schouleur, M., Schouler, C. and Blanco, J. (2013) Poultry as Reservoir for Extrain-testinal Pathogenic Escherichia coli O45:K1:H7-B2-ST95 in Humans. Veterinary Microbiology, 167, 506-512.
http://dx.doi.org/10.1016/j.vetmic.2013.08.007
[6] Guenther, S., Grobbel, M., Lübke-Becker, A., Goedecke, A., Friedrich, N.D., Wieler, L.H. and Ewers, C. (2010) Antimicrobial Resistance Profiles of Escherichia coli from Common European Wild Bird Species. Veterinary Microbiology, 144, 219-225.
http://dx.doi.org/10.1016/j.vetmic.2009.12.016
[7] Guenther, S., Ewers, C. and Wieler, L.H. (2011) Extended-Spectrum Beta-Lactamases Producing E. coli in Wildlife, yet Another Form of Environmental Pollution? Frontiers in Microbiology, 2, 246.
http://dx.doi.org/10.3389/fmicb.2011.00246
[8] Guenther, S., Aschenbrenner, K., Stamm, I., Bethe, A., Semmler, T., Stubbe, A., Stubbe, M., Batsajkhan, N., Glupczynski, Y., Wieler, L.H. and Ewers, C. (2012) Comparable High Rates of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli in Birds of Prey from Germany and Mongolia. PLoS ONE, 7, e53039.
http://dx.doi.org/10.1371/journal.pone.0053039
[9] Guinèe, P.A.M., Jansen, H.W., Wadström, T. and Sellwood, R. (1981) Escherichia coli Associated with Neonatal Diarrhoea in Piglets and Calves. In: de Leeww, P.W. and Guinèe, P.A.M., Eds., Laboratory Diagnosis in Neo-Natal Calf and Pig Diarrhoea: Current Topics in Veterinary and Animal Science, Vol. 13, Martinus Nijhoff Pub, The Netherlands, 126-162.
http://dx.doi.org/10.1007/978-94-009-8328-1_18
[10] Clermont, O., Bonacorsi, S. and Bingen, E. (2000) Rapid and Simple Determination of the Escherichia coli Phylogenetic Group. Applied and Environmental Microbiology, 66, 4555-4558.
http://dx.doi.org/10.1128/AEM.66.10.4555-4558.2000
[11] Clinical Laboratory Standards Institute (CLSI) (2011) Performance Standards for Antimicrobial Susceptibility Testing: Twenty-First Informational Supplement. CLSI Document M100-S21. National Committee for Clinical Laboratory Standards, CLSI, Wayne.
[12] Mora, A., López, C., Dabhi, G., Blanco, M., Blanco, J.E., Alonso, M.P., Herrera, A., Mamani, R., Bonacorsi, S., Moulin-Schouleur, M. and Blanco, J. (2009) Extraintestinal Pathogenic Escherichia coli O1:K1:H7/NM from Human and Avian Origin: Detection of Clonal Groups B2 ST95 and D ST59 with Different Host Distribution. BMC Microbiology, 9, 132.
http://dx.doi.org/10.1186/1471-2180-9-132
[13] Johnson, J.R., Murray, A.C., Gajewski, A., Sullivan, M., Snippes, P., Kuskowski, M.A. and Smith, K.E. (2003) Isolation and Molecular Characterization of Nalidixic Acid-Resistant Extraintestinal Pathogenic Escherichia coli from Retail Chicken Products. Antimicrobial Agents and Chemotherapy, 47, 2161-2168.
http://dx.doi.org/10.1128/AAC.47.7.2161-2168.2003
[14] Blanco, J.E., Blanco, M., Mora, A. and Blanco, J. (1997) Prevalence of Bacterial Resistance to Quinolones and Other Antimicrobials among Avian Escherichia coli Strains Isolated from Septicemic and Healthy Chickens in Spain. Journal Clinical Microbiology, 35, 2184-2185.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.