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Pitfalls and Uncertainties of Using Potentiometric Titration for Estimation of Plant Roots Surface Charge and Acid-Base Properties

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DOI: 10.4236/ajps.2014.513200    3,416 Downloads   4,475 Views   Citations

ABSTRACT

Amount and properties of roots surface charge are important for nutrient uptake and balance in plants. Roots surface charge markedly varies at different rizosphere conditions (particularly pH and ionic strength), which can markedly alter during vegetation season. Among recently available measuring methods, surface charge-pH dependence of roots (as well as other biological objects) is most easily evaluated by potentiometric titration. Use of this method is also easy at different ionic strengths. Potentiometric titration also allows for estimation of the distribution of charge generating surface groups. However, many applications of this method seem to be based either on incorrect methodical or theoretical approaches. In this paper we discuss the methodical and theoretical backgrounds of the titration method. Basing on experimental titration curves of roots of barley grown in nutrient solution, we show inconsistency of surface charge results obtained at different measuring conditions. Limitations of theoretical interpretations of the results are outlined also.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Jozefaciuk, G. , Szatanik-Kloc, A. , Lukowska, M. and Szerement, J. (2014) Pitfalls and Uncertainties of Using Potentiometric Titration for Estimation of Plant Roots Surface Charge and Acid-Base Properties. American Journal of Plant Sciences, 5, 1862-1876. doi: 10.4236/ajps.2014.513200.

References

[1] Ginn, B.R., Szymanowski, J.S. and Fein, J.B. (2008) Metal and Proton Binding onto the Roots of Fescue Rubra. Chemical Geology, 253, 130-135.
http://dx.doi.org/10.1016/j.chemgeo.2008.05.001
[2] Cheng, T. and Allen, H.E. (2001) Prediction of Uptake of Copper from Solution by Lettuce Lactuca sativa Romance). Environmental Toxicology & Chemistry, 20, 2544-2551.
http://dx.doi.org/10.1897/1551-5028(2001)020<2544:POUOCF>2.0.CO;2
[3] Meychik, N.R., Honarmand, S.J., Nikolaeva, Y.I. and Yermakov, I.P. (2007) Ion Exchange Properties of Cicer arietinum L. Root Cell Walls under Different Environmental Salt Conditions. Biologija, 53, 75-79
[4] Meychik, N.R. and Yermakov, I.P. (2001) Ion Exchange Properties of Plant Root Cell Walls. Plant and Soil, 234, 181-193.
http://dx.doi.org/10.1023/A:1017936318435
[5] Sentenac, H. and Grignon, C. (1981) A Model For Predicting Ionic Equilibrium Concentrations in Cell Walls. Plant Physiology, 68, 415-419.
http://dx.doi.org/10.1104/pp.68.2.415
[6] Kaulbach, E.S., Szymanowski, J.E.S. and Fein, J.B. (2005) Surface Complexation Modeling of Proton and Cd Adsorption onto an Algal Cell Wall. Environental Science and Technology, 39, 4060-4065.
http://dx.doi.org/10.1021/es0481833
[7] Wu, Y. and Hendershot, W.H. (2009) Cation Exchange Capacity and Proton Binding Properties of Pea (Pisum sativum L.) Roots. Water, Air, and Soil Pollution, 200, 353-369.
http://dx.doi.org/10.1007/s11270-008-9918-2
[8] Zhou, Z., Zhou, J., Wang, H. and Wang, J. (2008) Preparation and Surface Characteristics of Root Cell Walls of Soybean and Maize Seedlings. Plant Nutrition and Fertilizer Science, 14, 392-397.
[9] Jozefaciuk, G. and Lukowska, M. (2013) New Method for Measurement of Plant Roots Specific Surface. American Journal of Plant Sciences, 4, 1088-1094.
http://dx.doi.org/10.4236/ajps.2013.45135
[10] Lukowska, M. and Jozefaciuk, G. (2013) Unknown Mechanism of Plants Response to Drought: Low Soil Moisture and Osmotic Stresses Induce Severe Decrease in CEC and Increase in Acidity of Barley Roots. Journal of Agricultural Science, 5, 204-213
[11] Graham, E.R. and Baker, W.L. (1991) Ionic Saturation of Plant Roots with Special Reference to Hydrogen. Soil Science, 72, 435-442.
http://dx.doi.org/10.1097/00010694-195112000-00003
[12] Epstein, E. and Leggett, J.E. (1954) The Absorption of Alkaline Earth Cations by Barley Roots. Kinetics and Mechanism. American Journal of Botany, 41, 785-791. http://dx.doi.org/10.2307/2438542
[13] Ram, L.C. (1980) Cation Exchange Capacity of Plant Roots in Relation to Nutrients Uptake by Shoot and Grain as Influenced by Age. Plant and Soil, 55, 215-224. http://dx.doi.org/10.1007/BF02181801
[14] Rengel, Z. and Robinson, D.L. (1989) Aluminum and Plant Age Effects on Adsorption of Cations in the Donnan Free Space of Ryegrass Roots. Plant and Soil, 116, 223-227.
http://dx.doi.org/10.1007/BF02214551
[15] Grignon, C. and Sentenac, H. (1991) pH and Ionic Conditions in the Apoplast. Annual Review of Plant Physiology and Plant Molecular Biology, 42, 103-128.
http://dx.doi.org/10.1146/annurev.pp.42.060191.000535
[16] Shomer, I., Novacky, A.J., Pike, S.M., Yermiyahu, U. and Kinraide, T.B. (2003) Electrical Potentials of Plant Cell Walls in Response to the Ionic Environment. Plant Physiology, 133, 411-422. http://dx.doi.org/10.1104/pp.103.024539
[17] Kinraide, T.B. (2004) Possible Influence of Cell Walls upon Ion Concentrations at Plasma Membrane Surfaces. Toward a Comprehensive View of Cell-Surface Electrical Effects upon Ion Uptake, Intoxication, and Amelioration. Plant Physiology, 136, 3804-3813.
http://dx.doi.org/10.1104/pp.104.043174
[18] Kinraide, T.B. and Wang, P. (2010) The Surface Charge Density of Plant Cell Membranes (σ): An Attempt to Resolve Conflicting Values for Intrinsic σ. Journal of Experimental Botany, 61, 2507-2518.
http://dx.doi.org/10.1093/jxb/erq082
[19] Bartlett, R.J. (1964) Measurement of Cation- and Anion-Exchange Capacities of Roots Using NaCl Exchange. Soil Science, 98, 351-357.
http://dx.doi.org/10.1097/00010694-196412000-00001
[20] Vedy, J.C. and Bruckert, S. (1982) Soil Solution; Composition and Pedogenic Significance. In: Bonneau, M. and Souchier, B., Eds., Constituents and Properties of Soils, Academic Press, New York, 184-214.
[21] Zabowski, D. (1989) Limited Release of Soluble Organics from Roots during the Centrifugal Extraction of Soil Solutions. Soil Science Society of America Journal, 53, 977-979.
http://dx.doi.org/10.2136/sssaj1989.03615995005300030058x
[22] Arsova, A. (2002) Cation-Exchange Characteristics of Wheat, Barley and Pea Depending on the Osmotic Pressure in Nutrient Solutions of Low pH. Bulgarian Journal of Plant Physiology, 28, 35-45.
[23] Van De Geijn, S.C. and Petit, C.M. (1979) Transport of Divalent Cations: Cation Exchange Capacity of Intact Xylem Vessels. Plant Physiology, 64, 954-958.
http://dx.doi.org/10.1104/pp.64.6.954
[24] Korner, L., Moller, I.M., Kjellbom, P. and Larsson, C. (1984) Surface Properties of Plasmalemma Vesicles from Barley Roots and Shoots. 4th Congress of FESPP, Strasbourg, 29 July-3 August 1984, 515-516.
[25] Akerlund, H.E., Andersson, B., Persson, A. and Albertsson, P.A. (1979) Isoelectric Points of Spinach Thylakoid Membrane Surfaces as Determined by Cross Partition. Biochimica et Biophysica Acta, 552, 238-246.
http://dx.doi.org/10.1016/0005-2736(79)90280-3
[26] Crooke, W.M. (1964) The Measurement of the Cation-Exchange Capacity of Plant Roots. Plant and Soil, 21, 43-49.
http://dx.doi.org/10.1007/BF01373871
[27] McBride, M. (1997) A Critique of Diffuse Double Layer Models Applied to Colloid and Surface Chemistry. Clays and Clay Minerals, 45, 598-608.
http://dx.doi.org/10.1346/CCMN.1997.0450412
[28] Marschner, H. and Romheld, V. (1983) In Vivo Measurement of Root-Induced pH Changes at the Soil-Root Interface: Effect of Plant Species and Nitrogen Source. Zeitschrift für Pflanzenphysiologie, 111, 249-251.
[29] Barczi, A., Moller, I.M., Lundborg, T. and Kylin, A. (1984) The Surface Charge Density of Wheat Root Membranes. Physiologia Plantarum, 61, 535-540.
http://dx.doi.org/10.1111/j.1399-3054.1984.tb05166.x
[30] Moller, I.M., Lundborg, T. and Barczi, A. (1984) The Negative Surface Charge Density of Plasmalemma Vesicles from Wheat and Oat Roots. FEBS Letters, 167, 181-185. http://dx.doi.org/10.1016/0014-5793(84)80857-1
[31] Dahlin, C. (2003) Surface Charge Densities and Membrane Fluidities in Thylakoids with Different Degrees of Thylakoid Appression after Norflurazon Treatment. Photosynthetica, 41, 635-639.
http://dx.doi.org/10.1023/B:PHOT.0000027532.55335.a7
[32] Korner, L.E., Kjellbom, P., Larsson, C. and Moller, I.M. (1985) Surface Properties of Right Side-Out Plasma Membrane Vesicles Isolated from Barley Roots and Leaves. Plant Physiology, 79, 72-79.
http://dx.doi.org/10.1104/pp.79.1.72

  
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