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Enhanced aquaporin 8 expression after subtotal colectomy in rat

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DOI: 10.4236/ojgas.2013.35043    2,920 Downloads   4,297 Views   Citations

ABSTRACT

Background: Aquaporins (AQPs), the family of water-selective channels, are localized in various organs and tissues, including the gastrointestinal (GI) tract. However, the roles of AQPs in the GI tract remain unclear. Materials and Methods: Male SD rats were subjected to subtotal colectomy (Group C, n = 22) or a sham operation (Group S, n = 16) and were sacri-ficed on postoperative days 7, 14, and 28. Total RNAs from the distal ileum and rectum were extracted. Quantitative RT-PCR was performed to measure AQP8 mRNA expression. For light-microscopy or immunohistochemistry, paraffin-embedded sections of 4 μm were prepared with H-E staining or anti-AQP8 antibody reaction. Mann-Whitney U-test was performed to compare the AQP8 distributions between the two groups, and the statistical significance was defined as p < 0.05. Results: AQP8 mRNA expression was enhanced in both the ileum and rectum in Group C at day 7. AQP8 protein expression was consistently observed in the ileum and rectum. The villus length in the ileum of Group C was significantly greater than that of Group S at days 7 and 14. Conclusion: Enhanced AQP8 mRNA expression in the subtotal colectomy model suggests that AQP8 plays an important role in maintaining the intestinal fluid balance.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Nakano, M. , Koyama, Y. , Nogami, H. , Yamamoto, T. and Wakai, T. (2013) Enhanced aquaporin 8 expression after subtotal colectomy in rat. Open Journal of Gastroenterology, 3, 253-258. doi: 10.4236/ojgas.2013.35043.

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