Share This Article:

Optimisation of beef tenderisation treated with bromelain using response surface methodology (RSM)

Abstract Full-Text HTML Download Download as PDF (Size:258KB) PP. 65-72
DOI: 10.4236/as.2013.45B013    3,154 Downloads   4,653 Views   Citations

ABSTRACT

The purpose of this study is to determine the optimum condition for the tenderization of beef by bromelain using Response Surface Methodology (RSM). Initially, bromelain powder was produced from pineapple crown of variety N36. Production of the bromelain powder involves several process steps such as extraction, purification, desalting and freeze drying. The cube size beef of round part was treated with bromelain at different pHs of beef, immersion temperatures, bromelain solution concentrations, and immersion times according to the experimental design which was recommended by RSM of MINITAB software version 15. Beef tenderness was then measured by Texture Analyser. The MINITAB software Version 15 was used to optimise the tenderisation of beef by bromelain. The determination coefficient R2 was 99.97% meaning that the experimental data were acceptable. It was found that beef could be optimize tenderised 89.907% at the optimum condition at pH of beef of 5.4, immersion temperature of60℃, bromelain solution concentration of 0.1682% and immersion time of 10 minutes. The verification value of beef ten-derisation at the feasible optimum condition which was determined by experiment was 89.571%. Since the difference between the veri- fication and predicted values was less than 5%, therefore, the optimum condition for the tenderisation of beef predicted by MINITAB software Version 15 could be accepted.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Zainal, S. , Nadzirah, K.Z. , Noriham, A. and Normah, I. (2013) Optimisation of beef tenderisation treated with bromelain using response surface methodology (RSM). Agricultural Sciences, 4, 65-72. doi: 10.4236/as.2013.45B013.

References

[1] Maiti, A.K., Ahlawat, S.S., Sharma, D.P. and Khanna, N. (2008) Application of natural tenderisers in meat - A review. Agricultural Reviews, 29(3), 226-230.
[2] Deatherage, F.E.(1974) New approaches to the marketing of red meats. New protein food, Academic Press, New York. doi:10.1016/B978-0-12-054801-9.50012-0
[3] Ζochowska-Kujawska, J., Lachowics, K. and Sobczak, M. (2010). The tenderisation of wild boar meat using a calcium chloride, kefir, wine and pineapple marinade. Electronic Journal of Polish Agricultural Universities, 13, 1-8.
[4] Umesh Hebbar, H., Sumana, B. and Raghavarao, K.S.M. S.(2008) Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes. Bioresource Technology, 99, 4896-4902. doi: 10.1016/j.biortech.2007.09.038
[5] Hale, L.P., Greer, P.K., Trinh, C.T. and James, C.L. (2005) Proteinase activity and stability of natural bromelain preparations. International Immunopharmacology, 5(4), 783-793. doi: 10.1016/j.intimp.2004.12.007
[6] Ngampanya, B. and Phongtongpasuk, S. ( 2006) Effects of sucrose concentration on crude bromelain production of in vitro culture of pineapple (Ananas comosus var. ‘Pattavia’). Kasetsart Journal Natural Science, 40, 129 -134.
[7] Devakate, R.V., Patil, V.V., Waje, S.S. and Thorat, B.N. (2009) Purification and drying of bromelain. Separation and Purification Technology, 64, 259-264. doi: 10.1016/j.seppur.2008.09.012
[8] Ke, S., Huang, Y., Decker, E.A. and Hultin, H.O. (2009). Impact of citric acid on the tenderness, microstructure and oxidative stability of beef muscle. Meat Science, 82(1), 113-118. doi: 10.1016/j.meatsci.2008.12.010
[9] Jerez, N.C., Calkins, C.R. and Velazco, J. (2003) Prerigor injection using glycolytic inhibitors in low-quality beef muscles. Journal of Animal Science, 81, 997- 1003.
[10] Ketnawa, S., Sai-Ut, S., Theppakorn, T., Chaiwut, P. and Rawdkuen, S. (2009) Partitioning of bromelain from pineapple peel (Nang Lae cultv.) by aqueous two phase system. Asian Journal of Food and Agro-Industry, 2, 457-468.
[11] Ketnawa, S., Chaiwut, P. and Rawdkuen, S. (2011) Aqueous two-phase extraction of bromelain from pineapple peels (‘Phu Lae’ cultv.) and its biochemical properties. Food Science and Biotechnology, 20, 1219-1226.
[12] Tripathi, A. and Srivastava, S.K. (2012) Biodegradation of orange G by a novel isolated bacterial strain Bacillus megaterium ITBHU01 using Response Surface Methodology. African Journal of Biotechnology, 11, 1768- 1781.
[13] Jeong, G.T., Yang, H.S. and Park, D.H. (2009) Optimisation of transesterification of animal fat ester. Bioresource Technology, 100(1), 25-30.
[14] Sharma, S., Malik, A. and Satya, S. (2009) Application of response surface methodology (RSM) for optimisation of nutrient supplementation for Cr (V1) removal by As- pergillus lentulus AML05. Journal of Hazardous Materials, 164, 1198-1204.
[15] Cepeda, E.A. and Calvo, B. (2008) Sunflower oil hydrogenation: Study using response surface methodology. Journal of Food Engineering, 89(4), 370-374. doi: 10.1016/j.jfoodeng.2008.01.012
[16] Siti Mazlina, M.K., Abdul Ghani, L.A., Nur ‘Aliaa, A.R., Siti Aslina, H. and Rozita, O. (2008) Comparison on optimisation of star fruit juice using RSM between two Malaysian star fruit varieties (B11 and B10). Pertanika Journal of Science and Technology, 16, 1-13.
[17] Kongkaew, A., Usansa, U. and Wanapu, C. (2012) Optimisation of wort production from rice malt using enzymes and barley malt. African Journal of Biotechnology, 11, 9941-9949.
[18] Mullai, P.N., Syed Ali F. and Eldon R.R. (2010) Statistical analysis of main and interaction effects to optimize xylanase production under submerged cultivation conditions. Journal of Agricultural Science, 2, 144-153.
[19] Thanapimmetha, A., Vuttibunchon, K., Saisriyoot, M. and Srinophakun, P. (2011) Chemical and microbial hydrolysis of sweet sorghum bagasse for ethanol production. World Renewable Energy Congress-Sweden, Linköping, Sweden. 8-13 May 2011, 1, 389-396.
[20] Rengadurai, S., Preetha, B. and Viruthagiri, T. (2012) Response surface technique for optimisation of parameters for decolorization of Reactive red BS using Trametes hiruta. International Journal of ChemTech Research, 4, 21-28.
[21] Sudamalla, P., Saravanan, P. and Matheswaran, M. (2012) Optimisation of operating parameters using response surface methodology for adsorption of crystal violet by activated carbon prepared from mango kernel. Sustainable Environment Research, 22, 1-7.
[22] Sudamalla, P., Saravanan, P. and Matheswaran, M. (2012) Optimisation of operating parameters using response surface methodology for adsorption of crystal violet by activated carbon prepared from mango kernel. Sustainable Environment Research, 22, 1-7.
[23] Fang, X.L., Feng, J.T., Zhang, W.G., Wang, Y.H. and Zhang, X. (2010) Optimization of growth medium and fermentation conditions for improved antibiotic activity of Xenorhabdus nematophila TB using a statistical approach. African Journal of Biotechnology, 9, 8068-8077. doi: 10.5897/ajb09.1939
[24] Hismath, I., Wan Aida, W.M. and Ho, C.W. (2011) Optimisation of extraction conditions for phenolic compounds from neem (Azadirachta indica) leaves. International Food Research Journal, 18, 931-939.
[25] Prakash. O, Talat, M., Hasan, S.H. and Pandey, R.K. (2008) Factorial design for the optimisation of enzymatic detection of cadmium in aqueous solution using immobilised urease from vegetable waste. Bioresource Technology, 99, 7565-7572.
[26] Panwal, J.H., Viruthagiri, T. and Baskar, G. (2011) Statistical modeling and optimization of enzymatic milk fat splitting by soybean lecithin using response surface methodology. International Journal of Nutrition and Metabolism, 3, 50-57.
[27] Datta, D. and Kumar, S. (2012) Modeling and optimisation of recovery process of glycolic acid using reactive extraction. International Journal of Chemical Engineering and Applications, 3, 141-146. doi: 10.7763/ijcea.2012.v3.175
[28] Patel, S., Kothari, D. and Goyal, A. (2011) Enhancement of dextransucrase activity of Pediococcus pentosaceus SPAm1 by Response Surface Methodology. Indian Journal of Biotechnology, 10, 346-351.
[29] Noordin, M.Y., Venkatesh, V.C., Sharif, S., Elting, S. and Abdullah, A. (2004) Application of response surface methodology in describing the performance of coated carbide tools when turning AISI 1045 steel. Journal of Materials Processing Technology, 145, 46-58. doi: 10.1016/s0924-0136(03)00861-6
[30] Trinh, T.K. and Kang, L.S. (2010) Application of response surface method as an experimental design to optimise coagulation tests. Environmental Engineering Research, 15, 63-70. doi: 10.4491/eer.2010.15.2.063
[31] Yu, S.-J., Zhang, Y., Li, C.-R., Zhang, Q., Ma, Z.-Y. and Fan, M.-Z. Optimisation of ultrasonic extraction of mycelia polysaccharides from Paecilomyces hepiali using response surface methodology and its antioxidant activity. African Journal of Biotechnology, 10, 17241-17250.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.