Share This Article:

Construction and Analysis of SSH-cDNA Library from Leaves of Susceptible Rubber Clone Resistant to Powdery Mildew Induced by BTH

Abstract Full-Text HTML XML Download Download as PDF (Size:582KB) PP. 528-534
DOI: 10.4236/ajps.2013.43068    3,979 Downloads   5,645 Views   Citations

ABSTRACT

To understand the mechanism of benzothiadiazole (BTH)-induced susceptible rubber clone resistance to powdery mildew on gene level, a differentially expressed cDNA library was constructed by suppression subtractive hybridization (SSH) with rubber Reyan 7-33-97 clone. The constructed cDNA library was high integrity through detection of the critical processes of SSH, such as efficiency of adaptor connection, subtraction and conversion, as well as the type of recombinant genes. The positive rate was 99% after identification with random 400 white spots. The size of the cDNA clone inserted fragments was various but most in 400 bp - 1000 bp. There were 23 cDNA sequences matching the function of energy and basic metabolism, signal transduction, membrane and transport, secondary metabolism and so on after detection of the 42 positive clone sequences selected randomly from the cDNA library and comparison on nucleic acid sequences in Genbank. 7 ESTs were logged in Genbank and accession numbers were GW873071 and GW874604- GW874610. The results implicated that BTH could effectively induced rubber tree resistance to powdery mildew through increasing expresses of defense-related genes in leaves of rubber tree susceptible clone. It should provide a new approach for rubber disease management.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

C. Luo, Z. Fan, Y. Shen, X. Li, H. Chang, Q. Huang and L. Liu, "Construction and Analysis of SSH-cDNA Library from Leaves of Susceptible Rubber Clone Resistant to Powdery Mildew Induced by BTH," American Journal of Plant Sciences, Vol. 4 No. 3, 2013, pp. 528-534. doi: 10.4236/ajps.2013.43068.

References

[1] G. C. Mondal and K. Jacob, “Effect of Powdery Mildew Disease on Yield of Rubber in Northern Part of West Bengal,” Proceedings of Placrosym, 2002, pp. 531-534.
[2] J. Liu, “Recent Advances in Rubber Powdery Mildew Research,” Tropical Agricultural Science & Technology, Vol. 33, No. 3, 2010, pp. 1-5.
[3] J. L. Shan, Q. C. Xiao, Z. T. Yu, et al., “A Preliminary Study on Mechanism of Oligosaccharin Inducing Rubber Tree Resistance to Powdery Mildew,” Subtropical Plant Science, Vol. 34, No. 1, 2005, pp. 31-32. doi:10.1086/425207
[4] C. J. Luo, Z. W. Fan, Y. D. Shen, H. T. Cheng and L. Z. Liu, “Effects of Rubber Tree Resistance Induced by BTH to Oidium heveae and Assay of Resistance-Related Enzymes,” Chinese Journal of Tropical Crop, Vol. 32, No. 3, 2011, pp. 475-479.
[5] Z. Sun and F. C. Zheng, “Effect of BTH on Resistance Induction in Rubber against Colletotrichum gloeosporioides Disease,” Guangdong Agricultural Science, No. 7, 2008, pp. 76-77.
[6] J. Goerlach, S. Volrath, G. Knauf-Beiter, et al., “Benzothiadiazole, a Nevel Class of Inducers of Systemic Acquired Resistance, Activates Genes Expression and Disease Resistance in Wheat,” The Plant Cell, Vol. 8, 1996, pp. 629-643.
[7] C. Nombela, S. Pascual, M. Aviles, et al., “Benzothiadiazole Induces Local Resistance to Bemisia tabaci (Hemiptera: Aleyrodidae) in Tomato Plants,” Journal of Economic Entomology, Vol. 98, No. 6, 2005, pp. 2266-2271. doi:10.1603/0022-0493-98.6.2266
[8] B. Chinnasri, B. S. Sipes and D. P. Schmitt, “Effects of Acibenzolar-S-Methyl Application to Rotylenchulus reniformis and Meloidogyne javanica,” Journal of Nematology, Vol. 35, No. 1, 2003, pp. 110-114.
[9] Z. W. Fan, H. Buschmann and J. Sauerborn, “Main Effects and Interactions among Acibenzolar-S-Methyl, a Biocontrol Fungus and Sunflower Cultivar on Control of Orobanche cumana Wallr.,” Journal of Plant Diseases and Protection, Vol. 114, No. 2, 2007, pp. 76-81.
[10] L. Z. Xiong, M. W. Lee, M. Qi and Y. N. Yang, “Identification of Defense-Related Rice Genes by Suppression Subtractive Hybridization and Differential Screening,” Molecular Plant-Microbe Interaction, Vol. 14, No. 5, 2001, pp. 685-692. doi:10.1094/MPMI.2001.14.5.685
[11] K.-H. Kogel and G. Langen, “Induced Disease Resistance and Gene Expression in Cereals,” Cellular Microbiology, Vol. 7, No. 11, 2005, pp. 1555-1564. doi:10.1111/j.1462-5822.2005.00592.x
[12] C. Bovie, M. Ongena, P. Thonart, et al., “Cloning and Expression Analysis of cDNAs Corresponding to Genes Activated in Cucumber Showing Systemic Acquired Resistance after BTH Treatment,” BMC Plant Biology, Vol. 4, 2004, pp. 15-26. doi:10.1186/1471-2229-4-15
[13] X. H. Qiu, P. Z. Guan, M.-L. Wang, et al., “Identification and Expression Analysis of BTH Induced Genes in Papaya,” Physiological and Molecular Plant Pathology, Vol. 65, 2004, pp. 21-30. doi:10.1016/j.pmpp.2004.11.004
[14] B. De Nardi, R. Dreos, L. Del Terra, et al., “Differential Responses of Coffea arabica L. Leaves and Roots to Chemically Induced Systemic Acquired Resistance,” Genome, Vol. 49, 2006, pp. 1594-1605. doi:10.1139/g06-125
[15] J. A. Verica, S. N. Maximova, M. D. Strem, et al., “Isolation of ESTs from Cacao (Theobroma cacao L.) Leaves Treated with Inducers of the Defense Response,” Plant Cell Reports, Vol. 23, No. 6, 2004, pp. 404-413. doi:10.1007/s00299-004-0852-5
[16] S. C. Wang, “Preliminary Evaluation of Resistance of Novel Rubber Clones to Powdery Mildew,” Chinese Journal of Tropical Agriculture, Vol. 23, No. 5, 2003, pp. 1-4.
[17] Y. Yang, Z. L. Zhang, K. C. Liu, et al., “Clone and Characteristics of a Novel Gene HbUEP from Latex in Hevea brasiliensis,” Journal of Agricultural Biotechnology, Vol. 16, No. 2, 2008, pp. 305-308.
[18] B. Ouyang, T. Yang, H. X. Li, L. Zhang, Y. Y. Zhang, J. H. Zhang, Z. J. Fei and Z. B. Ye, “Identification of Early Salt Stress Response Genes in Tomato Root by Suppression Subtractive Hybridization and Microarray Analysis,” Journal of Experimental Botany, Vol. 58, No. 3, 2007, pp. 507-520. doi:10.1093/jxb/erl258
[19] K. Shirasu, T. Lahaye and M. W. Tan, “A Novel Class of Eukaryotic Zinc-Binding Protein Is Required for Disease Resistance Signaling in Barley and Development in C. elegans,” Cell, Vol. 99, No. 4, 1999, pp. 355-366. doi:10.1016/S0092-8674(00)81522-6
[20] P. R. Muskett, K. Kahn, M. J. Austin, et al., “Arabidopsis RARl Exerts Rate-Limiting Control of R Gene-Mediated Defenses against Multiple Pathogens,” The Plant Cell, Vol. 14, No. 5, 2002, pp. 979-992. doi:10.1105/tpc.001040
[21] C. Azevedo, A. Sadanandom, K. Kitagawa, et al., “The RARl Interactor SGT1: An Essential Component of R Gene Triggered Disease Resistance,” Science, Vol. 295, No. 5562, 2002, pp. 2073-2076. doi:10.1126/science.1067554
[22] S. Yamada, M. Katsuhara, W. B. Kelly, C. B. Michalowski and H. J. Bohnert, “A Family of Transcripts Encoding Water Channel Proteins: Tissue-Specific Expression in the Common Ice Plant,” The Plant Cell, Vol. 7, No. 8, 1995, pp. 1129-1142.
[23] C. Maurel, “Plant Aquaporins: Novel Functions and Regulation Properties,” FEBS Letters, Vol. 581, No. 12, 2007, pp. 2227-2236. doi:10.1016/j.febslet.2007.03.021
[24] H. W. Chuang, T. F. Hsieh, M. Duval and T. L. Thomas, “Genomic Analysis of Arabidopsis Gene Expression in Response to a Systemic Fungicide,” In: H. J. Bohnert and R. A. Prade, Eds., Genomics of Plants and Fungi, CRC Press, Boca Raton, 2003, pp. 237-253. doi:10.1201/9780203912249.ch7
[25] Y. J. Xia, H. Suzuki, J. Borevitz, J. Blount, Z. J. Guo, K. Patel, et al., “An Extracellular Aspartic Protease Functions in Arabidopsis Disease Resistance Signaling,” The EMBO Journal, Vol. 23, 2004, pp. 980-988. doi:10.1038/sj.emboj.7600086

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.