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The effect of Panton Valentin Leukocidin (PVL) on the airway smooth muscle viability

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DOI: 10.4236/ojmip.2013.31007    2,123 Downloads   4,469 Views  

ABSTRACT

Methicillin-resistant Staphylococcus aureus (MRSA) is recognized as a worldwide pathogen, and the incidence of community-acquired infections (CA-MRSA) is increased. A virulence factor has been found in most CA-MRSA infections, the Panton-Valentin leukocidin (PVL), which causes polymorphonuclear leukocytes lysis and acute uncontrolled inflammation and tissue injury. In this study we investigated the effect of bacterial supernatant of PVL positive or negative strains on airway smooth muscle obtained from rabbit trachea. MRSA that carry the PVL-gene, confirmed by PCR, is cultured on GP agar and colonies were transferred into casein casein yeast extract medium. The culture supernatants were removed after centrifugation and the presence of PVL was confirmed using an immunochromatographic test. Rabbit tracheal ASMC were isolated and incubated with PVL positive or negative bacterial supernatant (1:20 - 1:2000) for 1 - 3 days. The effect of PVL on the ASMC morphology or viability was estimated using microscope observations or indirect immunofluores- cence with anti-Smooth muscle α-actin antibody and Dapi for DNA staining, and Trypan blue staining, respectively. ASMC incubated with PVL exhibit increased cell size, granular cytoplasm, and ruptured nuclei. Furthermore, PVL reduces cell number mainly in ASMC incubated in the presence of 10% FBS, therefore actively proliferating cells. These results show that apart from the known effect of PVL on immune cells and inflammation process, PVL has a direct toxic effect on airway smooth muscle cells.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Stamatiou, R. , Petinaki, E. and Hatziefthimiou, A. (2013) The effect of Panton Valentin Leukocidin (PVL) on the airway smooth muscle viability. Open Journal of Molecular and Integrative Physiology, 3, 42-47. doi: 10.4236/ojmip.2013.31007.

References

[1] Pantosti, A. and Venditti, M. (2009) What is MRSA? European Respiratory Journal, 34, 1190-1196. doi:10.1183/09031936.00007709
[2] Dailiana, Z.H., Rigopoulos, N., Varitimidis, S.E., Poultsides, L., Petinaki, E. and Malizos, K.N. (2008) Clinical and epidemiological features of upper-extremity infections caused by Staphylococcus aureus carrying the PVL gene: A four-year study in Greece. Medical Science Monitor, 14, CR511-CR514.
[3] Chini, V., Petinaki, E., Foka, A., Paratiras, S., Dimitracopoulos, G. and Spiliopoulou, I. (2006) Spread of Staphylococcus aureus clinical isolates carrying PantonValentine leukocidin genes during a 3-year period in Greece. Clinical Microbiology and Infection, 12, 29-34. doi:10.1111/j.1469-0691.2005.01295.x
[4] Chini, V., Petinaki, E., Meugnier, H., Foka, A., Bes, M., Etienne, J., Dimitracopoulos, G. and Spiliopoulou, I. (2008) Emergence of a new clone carrying Panton-Valentine leukocidin genes and staphylococcal cassette chromosome mec type V among methicillin-resistant Staphylococcus aureus in Greece. Scandinavian Journal of Infectious Diseases, 40, 368-372. doi:10.1080/00365540701749230
[5] Vandenesch, F., Naimi, T., Enright, M.C., Lina, G., Nimmo, G.R., Heffernan, H., Liassine, N., Bes, M., Greenland, T., Reverdy, M.E. and Etienne, J. (2003) Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: Worldwide emergence. Emerging Infectious Diseases, 9, 978-984. doi:10.3201/eid0908.030089
[6] Adem, P.V., Montgomery, C.P., Husain, A.N., Koogler, T.K., Arangelovich, V., Humilier, M., Boyle-Vavra, S. and Daum, R.S. (2005) Staphylococcus aureus sepsis and the Waterhouse-Friderichsen syndrome in children. New England Journal of Medicine, 353, 1245-1251. doi:10.1056/NEJMoa044194
[7] Nastaly, P., Grinholc, M. and Bielawski, K.P. (2010) Molecular characteristics of community-associated methicillin-resistant Staphylococcus aureus strains for clinical medicine. Archives of Microbiology, 192, 603-617. doi:10.1007/s00203-010-0594-4
[8] Boyle-Vavra, S. and Daum, R.S. (2007) Communityacquired methicillin-resistant Staphylococcus aureus: The role of Panton-Valentine leukocidin. Laboratory Investigation, 87, 3-9. doi:10.1038/labinvest.3700501
[9] Shilo, N. and Quach, C. (2011) Pulmonary infections and community associated methicillin resistant Staphylococcus aureus: A dangerous mix? Paediatric Respiratory Reviews, 12, 182-189. doi:10.1016/j.prrv.2010.10.007
[10] Diep, B.A., Chan, L., Tattevin, P., Kajikawa, O., Martin, T.R., Basuino, L., Mai, T.T., Marbach, H., Braughton, K.R., Whitney, A.R., Gardner, D.J., Fan, X., Tseng, C.W., Liu, G.Y., Badiou, C., Etienne, J., Lina, G., Matthay, M.A., DeLeo, F.R. and Chambers, H.F. (2010) Polymorphonuclear leukocytes mediate Staphylococcus aureus Panton-Valentine leukocidin-induced lung inflammation and injury. Proceedings of the National Academy of Sciences of the USA, 107, 5587-5592. doi:10.1073/pnas.0912403107
[11] Badiou, C., Dumitrescu, O., George, N., Forbes, A.R., Drougka, E., Chan, K.S., Ramdani-Bouguessa, N., Meugnier, H., Bes, M., Vandenesch, F., Etienne, J., Hsu, L.Y., Tazir, M., Spiliopoulou, I., Nimmo, G.R., Hulten, K.G. and Lina, G. (2010) Rapid detection of Staphylococcus aureus Panton-Valentine leukocidin in clinical specimens by enzyme-linked immunosorbent assay and immunochromatographic tests. Journal of Clinical Microbiology, 48, 1384-1390. doi:10.1128/JCM.02274-09
[12] Chachami, G., Hatziefthimiou, A., Liakos, P., Ioannou, M.G., Koukoulis, G.K., Bonanou, S., Molyvdas, P.A., Simos, G. and Paraskeva, E. (2007) Exposure of differentiated airway smooth muscle cells to serum stimulates both induction of hypoxia-inducible factor-1α and airway responsiveness to ACh. American Journal of Physiology— Lung Cellular and Molecular Physiology, 293, L913-922. doi:10.1152/ajplung.00459.2006
[13] Stamatiou, R., Paraskeva, E., Boukas, K., Gourgoulianis, K.I., Molyvdas, P.A. and Hatziefthimiou, A.A. (2009) Azithromycin has an antiproliferative and autophagic effect on airway smooth muscle cells. European Respiratory Journal, 34, 721-730. doi:10.1183/09031936.00089407
[14] Lo, W.T. and Wang, C.C. (2011) Panton-Valentine leukocidin in the pathogenesis of community-associated methicillin-resistant Staphylococcus aureus infection. Pediatrics & Neonatology, 52, 59-65. doi:10.1016/j.pedneo.2011.02.008
[15] Kahl, B.C. (2010) Impact of Staphylococcus aureus on the pathogenesis of chronic cystic fibrosis lung disease. International Journal of Medical Microbiology, 300, 514519. doi:10.1016/j.ijmm.2010.08.002
[16] Gerogianni, I., Mpatavanis, G., Gourgoulianis, K., Maniatis, A., Spiliopoulou, I. and Petinaki, E. (2006) Combination of staphylococcal chromosome cassette SCCmec type V and Panton-Valentine leukocidin genes in a methicillin-resistant Staphylococcus aureus that caused necrotizing pneumonia in Greece. Diagnostic Microbiology and Infectious Disease, 56, 213-216. doi:10.1016/j.diagmicrobio.2006.06.005
[17] L?ffler, B., Hussain, M., Grundmeier, M., Brück, M., Holzinger, D., Varga, G., Roth, J., Kahl, B.C., Proctor, R.A. and Peters, G. (2010) Staphylococcus aureus panton-valentine leukocidin is a very potent cytotoxic factor for human neutrophils. PLOS Pathogens, 6, e1000715. doi:10.1371/journal.ppat.1000715
[18] Genestier, A.L., Michallet, M.C., Prévost, G., Bellot, G., Chalabreysse, L., Peyrol, S., Thivolet, F., Etienne, J., Lina, G., Vallette, F.M., Vandenesch, F. and Genestier, L. (2005) Staphylococcus aureus Panton-Valentine leukocidin directly targets mitochondria and induces Bax-independent apoptosis of human neutrophils. Journal of Clinical Investigation, 115, 3117-3127. doi:10.1172/JCI22684
[19] Moskowitz, S.M. and Wiener-Kronish, J.P. (2010) Mechanisms of bacterial virulence in pulmonary infections. Current Opinion in Critical Care, 16, 8-12. doi:10.1097/MCC.0b013e3283354710
[20] Amrani, Y. and Panettieri, R.A. (2003) Airway smooth muscle: Contraction and beyond. International Journal of Biochemistry & Cell Biology, 35, 272-276. doi:10.1016/S1357-2725(02)00259-5
[21] Vignola, A.M., Mirabella, F., Costanzo, G., Di Giorgi, R., Gjomarkaj, M., Bellia, V. and Bonsignore, G. (2003) Airway remodeling in asthma. Chest, 123, 417S-422S. doi:10.1378/chest.123.3_suppl.417S-a
[22] Lazaar, A.L. and Panettieri, R.A. Jr. (2001) Airway smooth muscle as an immunomodulatory cell: A new target for pharmacotherapy? Current Opinion in Pharmacology, 1, 259-264. doi:10.1016/S1471-4892(01)00046-7
[23] Wannet, W.J., Spalburg, E., Heck, M.E., Pluister, G.N., Tiemersma, E., Willems, R.J., Huijsdens, X.W., de Neeling, A.J. and Etienne, J. (2005) Emergence of virulent methicillin-resistant Staphylococcus aureus strains carrying Panton-Valentine leucocidin genes in The Netherlands. Journal of Clinical Microbiology, 43, 3341-3345. doi:10.1128/JCM.43.7.3341-3345.2005

  
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