A simple and efficient seed-based approach to induce callus production from B73 maize genotype

Simeon O. Kotchoni, Pacôme A. Noumavo, Adolphe Adjanohoun, Daniel P. Russo, John Dell’Angelo, Emma W. Gachomo, Lamine Baba-Moussa

Centre de Recherches Agricoles, Institut National des Recherches Agricoles du Benin, Allada, Benin.
Département de Biochimie et Biologie Cellulaire, Université d’Abomey-Calavi, Cotonou, Benin.
Department of Biology, Rutgers, The State University of New Jersey, Camden, USA.

DOI: 10.4236/ajmb.2012.24039                

Abstract

The wild type maize genotype, B73, is not amenable for callus production and an efficient protocol for B73 maize callus induction has never been reported up-to-date. Scientific efforts in producing B73 maize callus using all known callus inducible media have been unsatisfactory. Here we developed and described an efficient protocol for callus induction from B73 maize seedlings. The protocol is based on well known callus inducible media CM4C where we have sequentially subtracted some chemical compounds and added some new compounds mediating cell proliferations. This newly described protocol was able to induce callus production in a wide range of crop species including rice and soybean. We found that cell proliferation factors, NAA (auxin analog) and 2,4 D (auxin influx carrier) were not only very crucial but required for positive B73 maize callus induction. The absence of one or the other will lead to the failure of B73 maize callus production. The well known CM4C callus induction composition lacks NAA. Our findings will advance genetic studies of maize mutants generated from B73 genotype background.

Keywords

B73 Maize Genotype; Soybean; Callus; CM4C; Maize; Protocol; Rice; NAA; 2, 4-D

Conflicts of Interest

The authors declare no conflicts of interest.

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