Isoform Inference From RNA-Seq Samples Based on Gene Structures on Chromosomes ()
Abstract
The emerging RNA-Seq technology makes it possible to infer splicing variants from millions of short sequence reads. Here we present a method to identify isoforms by their specific signatures on chromosomes including both exons and junctions. By applying this method to a RNA-Seq dataset of gastric cancer, we showed that our method is more accurate and sensitive than other isoform inference tools such as RSEM and Cufflinks. By constructing a network from gene list identified by our method but missed by other tools, we found that some cancer-related genes enriched in network modules have significant implications for cancer drug discovery.
Share and Cite:
Ji, Y. and Wei, J. (2013) Isoform Inference From RNA-Seq Samples Based on Gene Structures on Chromosomes.
Journal of Biosciences and Medicines,
1, 1-5. doi:
10.4236/jbm.2013.11001.
Conflicts of Interest
The authors declare no conflicts of interest.
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