TITLE:
The Effect of a Lactobacillus-Based Probiotic for the Control of Necrotic Enteritis in Broilers
AUTHORS:
Sherryll Lynn Layton, Xochitl Hernandez-Velasco, Shivaramaiah Chaitanya, Jorge Xavier, Anita Menconi, Juan David Latorre, Gopala Kallapura, Vivek Ayamchirakkunnel Kuttappan, Ross Elderon Wolfenden, Raphael Lucio Andreatti Filho, Billy Marshal Hargis, Guillermo Téllez
KEYWORDS:
Lactobacillus; Necrotic Enteritis; Salmonella; Coccidiosis; Probiotic
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.4 No.11A,
November
1,
2013
ABSTRACT:
Necrotic Enteritis (NE) caused by Clostridium perfringens (CP) in poultry is probably the most
important bacterial disease in terms of economic implications. The disease is
multi-factorial and is invariably associated with predisposing factors. The
present study investigated the effect of a commercially available Lactobacillus-based probiotic (FM-B11)
for the control of necrotic enteritis in broiler chickens. In experiment 1,
one-day-of-hatch broiler chicks were randomly allocated to the following
treatment groups: 1) Non-challenged (NC); 2) Challenged (C); 3) Challenged + probiotic (C+ FM-B11). Prior to placement, chicks in groups 2 and
3 received 0.25 mL of Salmonella typhimurium (ST) containing 105 cfu of viable cells by oral gavage. At 14, 15
and 16 days of age, all chicks in group 3 were treated with FM-B11 in the
drinking water at a concentration of 106 cfu/ml. At 21d of age, all
chicks in groups 2 and 3, were individually challenged with 5 × 104 sporulated oocysts of E. maxima by oral gavage. At 26d of age,
all chicks in groups 2 and 3, were individually challenged with 108 cfu CP; body weight (BW) was recorded prior to challenge. The experiment was terminated
at 29 days of age and the following parameters were evaluated: NE-associated
mortality, CP lesion scores, CP concentrations in ileum, BW, and body weight
gain (BWG). Chicks treated with FM-B11 had significantly (P 0.05) difference
in lesion score between C and C + FM-B11, group C + FM-B11 had significantly (P
Lactobacillus-based probiotic FM-B11 was
able to reduce the severities of NE, as a secondary bacterial infection, in an
experimental NE challenge model; as well as, in a commercial field outbreak of
NE.