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Rapid Detection Tool for Vancomycin-Resistant Enterococcus (VRE) Directly from Human Specimens

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DOI: 10.4236/aim.2014.412092    2,992 Downloads   3,506 Views  

ABSTRACT

A new, simple, and one-step tool for the direct detection of vancomycin-resistant Enterocococcus (VRE) EPI-V® (Pilots Point LLC, Sarasota, FL) is presented. It contains all the ingredients in a unique stable powder form in a standard test tube. One only needs to add water, inoculate the specimen, and incubate. Specimens consisted of 553 sequential human rectal/perirectal swabs for VRE surveillance. The presence of VRE was denoted by the production of two sequential color changes corresponding to growth in bile-esculin and production of a positive PYR reaction. The EPI-V® tool was compared to reference VRE detection methods. EPI-V® showed a sensitivity of  102% and a specificity of 98.4% for the detection of VRE. The EPI-V® tool offers significant advantages: no skilled technologist time required, simple quality control, highly conserved incubator and refrigerator space, and low cost.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Edberg, S. (2014) Rapid Detection Tool for Vancomycin-Resistant Enterococcus (VRE) Directly from Human Specimens. Advances in Microbiology, 4, 835-838. doi: 10.4236/aim.2014.412092.

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