Effective Method to Resolve the Chromosome Numbers in Pistacia Species (Anacardiaceae) - American Journal of Plant Sciences

AJPS > Vol.5 No.20, September 2014

American Journal of Plant Sciences

Volume 5, Issue 20 (September 2014)

ISSN Print: 2158-2742 ISSN Online: 2158-2750

Google-based Impact Factor: 1.20 Citations h5-index & Ranking

Effective Method to Resolve the Chromosome Numbers in Pistacia Species (Anacardiaceae) ()

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DOI: 10.4236/ajps.2014.520306 3,020 Downloads 3,621 Views Citations

Author(s)

Mohannad Al-Saghir^1*, Shadi Abu Baker², Robert Pusok¹

Affiliation(s)

¹Department of Environmental and Plant Biology, Ohio University Zanesville, Zanesville, OH, USA.
²Department of Chemistry, Ohio University Zanesville, Zanesville, OH, USA.

ABSTRACT

The genus Pistacia L. belongs to the Anacardiaceae family and includes at least eleven species. Cytogenetic studies addressing the genus Pistacia are rather few. Chromosome numbers of the different Pistacia species, revealed by these studies, are questionable due to the fact that poor chromosome counting protocols were used, and these protocols are hampered by the extremely small-sized chromosomes of Pistacia species. The aim of this study was to develop a more effective method to resolve the chromosome numbers in Pistacia species using a fluorescent microscope. The method described here is modified from the Sigma Plant Protoplast Digest/Wash Solution protocol. The method used here is highly effective for karyotyping analysis and studying population genetics of Pistacia species. Moreover, it is easy and can be reproduced for other species that have smaller chromosomes. This method can be used for plant herbarium specimens or field plants. This study provides valuable chromosomal data for cytogeneticists and plant breeders who are working on this genus. It provides additional insight into understanding the taxonomic and phylogenetic relationships among Pistacia species. The chromosomes described here are also suitable for gene and genome mapping.

KEYWORDS

Pistacia, Cytogenetics, Chromosome Numbers, Fluorescent Microscopy

Share and Cite:

Al-Saghir, M. , Baker, S. and Pusok, R. (2014) Effective Method to Resolve the Chromosome Numbers in Pistacia Species (Anacardiaceae). American Journal of Plant Sciences, 5, 2913-2916. doi: 10.4236/ajps.2014.520306.

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