A new risk assessment method for evaluation of oxidative chemicals using catalase mutant mouse primary hepatocytes ()
ABSTRACT
We examined the possibility of developing a new risk assessment method for potentially hazardous chemicals by using mouse primary hepatocytes from acatalasemic mice (Csb) and the wild-type (Csa) as predictive model. Chemical-induced cytotoxicities, such as hydrogen peroxide and lawsone, a main hair dye ingredient of henna, were examined. We observed the differences in cell survival between the Csa and Csb in a dose-dependant manner after treatment with either hydrogen peroxide or lawsone, supporting the usefulness of this newly established method for hazard identification of oxidative chemicals in a risk assessment process. More chemicals will be tested to confirm the usefulness of this method for the preliminary screening of oxidative chemicals before animal experimentation.
Share and Cite:
Wang, D. , Ishikawa, Y. , Miyazaki, M. , Fujita, H. , Tsutsui, K. , Sano, K. , Masuoka, N. and Ogino, K. (2011) A new risk assessment method for evaluation of oxidative chemicals using catalase mutant mouse primary hepatocytes.
Health,
3, 288-291. doi:
10.4236/health.2011.35050.
Cited by
No relevant information.