Patatin-like PLA2 with cytotoxicity against mammalian and plant tumour cells - Advances in Biological Chemistry

ABC > Vol.3 No.5, October 2013

Advances in Biological Chemistry

Volume 3, Issue 5 (October 2013)

ISSN Print: 2162-2183 ISSN Online: 2162-2191

Google-based Impact Factor: 0.5 Citations

Patatin-like PLA₂ with cytotoxicity against mammalian and plant tumour cells ()

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DOI: 10.4236/abc.2013.35053 3,201 Downloads 5,735 Views Citations

Author(s)

Bernard Griffaut, Eric Debiton, Marie-Josèphe Galmier, Ana Mustel, Jean-Claude Madelmont, Gérard Ledoigt

Affiliation(s)

Clermont Université, UMR 547 INRA-Université PIAF, Université Blaise-Pascal, Campus des Cézeaux, Aubière Cedex, France.
Clermont Université, UMR 547 INRA-Université PIAF, Université Blaise-Pascal, Campus des Cézeaux, Aubière Cedex, France Clermont Université, Université d’Auvergne, Imagerie Moléculaire et Thérapie Vectorisée, BP, Clermont-Ferrand, France.
Clermont Université, Université d’Auvergne, Imagerie Moléculaire et Thérapie Vectorisée, BP, Clermont-Ferrand, France Inserm, UMR 990, IMTV, Clermont-Ferrand, France.

ABSTRACT

Plants can respond to traumatism by synthesis and secretion of defence molecules. Wound-healing and desiccation stress of pieces of potato tuber parenchyma (Solanum tuberosum) promoted the secretion of a patatin-like phospholipase A2, PLA2 (EC.3.1.1.4.) that displayed cytotoxic activity against tumour cells. The potato secretion product, an oligomeric form of patatin-like protein, was shown to contain several isoforms of PLA2 polypeptides and to be associated with other proteins, including Kunitz-type protease inhibitors. Patatin-like protein secretion was inhibited by vanadate. Secreted patatin-like proteins displayed specific features, such as extracellular function and low molecular weights, mainly 36 to 40 kDa. The 36-kDa polypeptide sequence was related to iPLA2α. Polypeptide spots of secreted patatin-like protein exhibited a nucleotide-binding consensus motif, GGGIKG that has been described in iPLA2 gene family. The cytotoxic agent caused cell death of plant crown-gall induced by Agrobacterium tumefaciens, and inhibited B16 cell proliferation, but at the same concentration did not display any toxicity against non-transformed cells. PLA2 enzyme activity was required for cytotoxicity against B16 melanoma cells. A model for such a specific activity against tumour cells is discussed in connection with asymmetric phospholipid patterns of cell membranes. In conclusion, secreted patatin-like PLA2 (e-patatin) may represent a novel therapeutic target for the development of new agents against cancer.

KEYWORDS

Cytotoxicity; Patatin; Phospholipase A₂

Share and Cite:

Griffaut, B. , Debiton, E. , Galmier, M. , Mustel, A. , Madelmont, J. and Ledoigt, G. (2013) Patatin-like PLA₂ with cytotoxicity against mammalian and plant tumour cells. Advances in Biological Chemistry, 3, 485-500. doi: 10.4236/abc.2013.35053.

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