Monoculture of root-knot nematode, Meloi-
dogyne incognita race 2 was maintained on tomato plants.
Pure culture was multiplied and after processing the
number of larvae per ml, suspension was counted before
inoculation. The counting of nematode @ per ml of sus-
pension was done with the help of especially made
counting dish under the microscope. The species/race
was identified by using perineal pattern determination/
differential host range test Hartman and Sasser [17] be-
fore use.
The experiment was conducted under net house condi-
tions in the Department of Botany Aligarh Muslim Uni-
versity Aligarh (AMU) India during the year 2011.
Throughout the course of studies, 6 clay pots containing
1 Kg sterilized soil + river sand + farm yard manure
(3:1:1) were used and arranged in randomised complete
block design and five replicates of each treatment were
made and an untreated plant was served as control. The
plants were watered regularly. The experiment was ter-
minated after 45 days and plant growth parameters were
observed in terms of Plant length, fresh weight, dry
weight, number of fruits and fruit weight. Data obtained
were analysed statistically at P = 0.05 and P = 0.01%.
3. RESULTS AND DISCUSSION
Inoculums level of fungus Fusarium oxysporum f sp.
Lycopersici 0.25 g and 0.50 g/kg soil showed no signifi-
cant reduction in plant growth parameters viz. Plant
length, fresh and dry weight, number of fruits and fruit
weight. However, the plants inoculated with inoculum
levels 1.0 g - 8.0 g/kg soil showed significant reduction
in plant length (66.5 - 63.1 cm), fresh weight (179.1 -
174.8 gm), dry weight (33.3 - 29.3 g), number of fruits
(13.7 - 10.5) and fruit weight (234.6 - 225.0) respectively.
Similarly plant inoculated with 250 to 500 J2/kg soil of
Meloidogyne incognita race-2 showed no significant
reduction in plant growth para meters while plants inocu-
lated with 1000 - 8000 J2 show ed significant reduction in
plant growth parameters viz., Plant length (63.8 - 58.8
cm), Fresh weight (178.3 - 164.4 g), dry weight (31.7 -
23.6), number of fruits (11.8 - 8.1) and fruit weight (230.3 -
216.8) as compared to control (Uninoculated) Table 1.
Tabel 1. Effect of different inoculum levels of root-knot nematode, Meloidogyne incognita race 2 on plant growth parameters of to-
mato.
Length (cm) Fresh wt. (gm) Dry wt. (gm)
Treatments Shoot Root Total Shoot Root Total Shoot Root Total
No. of
fruits Fruit weight
(gm)
250N 49.1 24.7 73.8 138.6 62.2 200.8 25.2 13.3 38.5 17.7 267.2
500N 48.3 23.9 72.2 134.9 60.6 195.5 23.7 12.5 36.2 15.3 266.8
1000N 42.4 21.4 63.8 124.4 53.9 178.3 19.2 12.5 31.7 11.8 230.3
2000N 41.5 20.8 62.3 128.2 52.4 180.6 18.5 11.7 30.2 10.4 227.2
4000N 40.4 20.3 60.7 120.1 50.6 170.7 15.9 9.4 25.3 9.3 220.6
8000N 39.5 19.3 58.8 117.1 47.3 164.4 14.7 8.9 23.6 8.1 216.8
Control 49.8 25.2 75.0 141.3 63.5 204.8 26.1 13.6 39.7 19.2 268.8
LSD at 5% 5.26 16.25 2.45 1.20 21.20
LSD at 1% 7.78 23.03 3.41 1.69 28.14
Value are mean of five replicates.
Copyright © 2012 SciRes. OPEN ACCESS
S. Ansari et al. / Agricultural Sciences 3 (2012) 844-847
846
Table 2. Effect of different inoculum levels of fungus, Fusarium oxysporum, f sp. lycopersici on plant growth parameters of tomato.
Length (cm) Fresh wt. (gm) Dry wt. (gm)
Treatments Shoot Root Total Shoot Root Total Shoot Root Total
No. of
fruits Fruit wt.
(gm)
0.25F 49.6 25.0 74.6 140.9 63.3 204.2 25.4 13.4 38.8 18.4 268.2
0.50F 49.2 24.6 73.8 140.2 62.9 203.1 24.6 13.0 37.6 16.6 267.3
1.00F 43.5 23.0 66.5 123.9 55.2 179.1 21.1 11.2 33.3 13.7 234.6
2.00F 43.9 22.3 66.2 125.0 53.2 178.2 20.6 10.9 31.5 12.2 230.8
4.00F 42.0 22.3 64.3 122.4 53.1 175.5 19.7 10.4 30.1 11.1 227.1
8.00F 42.2 20.9 63.1 122.6 52.2 174.8 19.2 10.1 29.3 10.5 225.0
Control 49.8 25.2 75.0 141.3 63.5 204.8 26.1 13.6 39.7 19.2 268.8
LSD at 5% 5.44 16.94 2.22 1.28 22.54
LSD at 1% 8.03 24.25 3.71 1.80 30.01
Values are mean of five replicates.
0
20
40
60
80
100
120
140
160
180
T1 T2 T3 T4 T5 T6
Repr oductionfactor‐
Nu mberofGall/ro otsystem‐
Tre atmen ts
Figure 1. Reproduction factor and Number of galls/root system induced by different inocu-
lum levels of root-knot nematode, Meloidogyne incognita race 2 on tomato plants. T1 =
250 J2 of root-knot nematode, Meloidogyne incognita race 2; T2 = 500 J2; T3 = 1000 J2; T4
= 2000 J2; T5 = 4000 J2; T6 = 8000 J2.
Fusarium species is most important and soil borne
disease of tomato Jones et al. [18] and Smith et al. [19].
Fungal pathogen can surv ive in soil for several years and
cause infection in plants, Walker [20] Holiday [21].
Plants treated with M. incognita race 2 showed greatest
reductions in plant growth parameters in comparison to
plants treated with Fusarium oxysporum f sp. lycopersici
(Table 2).
A significant linear relationship was found between
the initial population (Pi) and the final population (Pf) of
M. incognita race 2. The multiplication of root-knot
nematode significantly reduced with the increase in the
inoculum levels. The reproduction factor was highest
(16.6%) at the minimum inoculums level 250 J2/kg soil
and lowest (2.6%) at the maximum inoculum level 8000
J2/plant. Thus, the rate of nematode multiplication show ed
a declining trend with the increase in th e initial inoculum
level suggesting it to be a density depending phenome-
non (Figure 1). Higher multiplication rate for low initial
population and low multiplication rate in high initial
population was observed. Same results were also ob-
served by Brker et al. [22], Di Vito and Ekanayake [23]
and Haseeb et al. [24]. It can be concluded from these
results that the damaging threshold levels of M. incognita
race 2 on tomato was found to be as 1000 J2/kg soil while
threshold level of fungus, Fusarium oxysporum f sp. Ly-
copersici, was 1 g/kg soli (Table 1 and Figure 1) .
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