TITLE:
Antioxidant Activity and Phytochemical Screening in Acacia rigidula Benth. Leaves
AUTHORS:
Aneth Ochoa-Negrete, Rene Rangel, Marcus Antonius Ynalvez, Ruby A. Ynalvez
KEYWORDS:
Acacia, Plant Extract, Antioxidant Activity, Total Phenolic Acid, HPLC, Gallic Acid
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.15 No.1,
January
26,
2024
ABSTRACT: This study aimed to compare the total phenolic
content (TPC) and antioxidant activities of A. rigidula extracts. It also aimed to
identify phenolic acids present in the extracts. The
2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS),
2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP),
and ferric thiocyanate lipid peroxidation antioxidant assays were performed.
High performance liquid chromatography was used to identify phenolic acids.
There was no solvent effect on TPC nor on scavenging
activities, and inhibition of lipid peroxidation (p > 0.05) among solvent
extracts. On the other hand, 1:1:3 water: acetone: methanol extract (10.22 mg
GAE/g sample) had significantly higher reducing potential than 50% ethanol
extract (EE) (9.259 mg GAE/g sample) (p 0.05). Phenolic fraction designated as fraction 4 had the highest
antioxidant activity (p reducing potential, 22.26 mg of GAE/g
sample. DPPH scavenging activities of fractions 4 (55.59%) and 5
(55.64%) were significantly higher than the other fractions (p A. rigidula extracts contain gallic, caffeic, vanillic, p-coumaric, salicylic acids and
vanillin.