TITLE:
Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis Methods
AUTHORS:
Rokhaya Gueye, Viviane Chatchueng Sandefo, Babacar Beye, Elhadji Ousmane Faye, Amadou Diop, Serigne Omar Sarr, Bara Ndiaye, Yérim Mbagnick Diop
KEYWORDS:
Poultry Feed, Aflatoxin, High Performance Liquid Chromatography, Thin-Layer Chromatography, Dakar
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.13 No.11,
November
30,
2022
ABSTRACT: Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs
variety. Ingestion of contaminated feed by poultry can lead to impaired health
and zootechnical performances but also a human diet safety problem related to
residues presence in animal origin products. Aflatoxin B1 contamination of
poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom,
Sangalkam) was studied. A total of 15 samples were collected from Dakar city
markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin
B1 quantification was performed by high performance liquid chromatography and thin-layer
chromatography. HPLC results showed that all samples were contaminated with
levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb
respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom
had an aflatoxin B1 level above the maximum
limit set by regulations. TLC is a suitable method for aflatoxins detection. However,
it was associated with overestimation for aflatoxin B1 quantification. Results
suggest that poultry feed represents a real
source of human diet contamination. In addition, HPLC remains the most reliable
quantification technique for quality control.