TITLE:
First Report of Pseudopestalotiopsis theae Causing Aloe vera Leaf Spot Disease in Bangladesh
AUTHORS:
Md. Sabbir Ahmmed, Md. Maniruzzaman Sikder, Satya Ranjan Sarker, Md. Sharif Hossain, Razib Datta Shubhra, Nuhu Alam
KEYWORDS:
Fungal Biology, Molecular, Growth Characters, Bio-Control Agents, Fungicides
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.13 No.2,
February
18,
2022
ABSTRACT: Aloe vera (L.) Burm.f. is one of the important medicinal
plants, has been commercially cultivated in the Northern part of Bangladesh. An
experiment was conducted to detect A.
vera leaf disease collected from Natore district, Bangladesh. Fungal leaf
spot disease caused by Pseudopestalotiopsis
theae (Sawada) Maharachch., K.D. Hyde & Crous was identified through
morphological features and sequencing of internal transcribed spacer region of
ribosomal DNA. After submitting nucleotide sequences to NCBI, we received an
accession number MH333081.1: Pseudopestalotiopsis
theae. The growth pattern of the isolated fungal pathogen was evaluated on
different solid culture media, at different temperature and light conditions.
The results showed the maximum mycelial growth of the fungus on the Richard
Agar medium under the complete dark condition at 25°C. We evaluated fungal
antagonists against the isolated pathogenic fungus, in which Trichoderma
asperellum showed optimistic
results. Synthetic fungicides—Tilt 250EC and Ridomil Gold completely inhibited the studied fungus’s vegetative growth. Pseudopestalotiopsis theae causing A. vera leaf spot disease in Bangladesh is a new record to the best of our knowledge.