TITLE:
Vitamins C, E, and NADH on in Vitro Lymphocyte Proliferation and Redox Status among Obese Patients
AUTHORS:
Asmaa Meraou, Hafida Merzouk, Amel Saidi, Amel Medjdoub, Sid Ahmed Merzouk, Slimane Belbraouet
KEYWORDS:
Obesity, Lymphocytes, Vitamins, Cytokines, Oxidant/Antioxidant Status
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.7 No.12,
October
17,
2016
ABSTRACT: Background: The prevalence of overweight and obesity associated with oxidative
stress and immune abnormalities is continuously increasing. Antioxidant supplementations
might counteract potential damage caused by ROS to cellular tissues.
Objective: To determine the role of vitamins on immune improvement during obesity,
we investigated in vitro effects of vitamins C, E, and NADH on mitogen-stimulated
proliferation, Th1- and Th2-type cytokine production, and oxidant/antioxidant status
of lymphocytes isolated from obese patients. Methods: Peripheral blood lymphocytes
were isolated using a density gradient of Histopaque. They were in vitro cultured
and stimulated by Con A in the presence or absence of vitamins. Cell proliferation
was determined by MTT assay and interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions. Cell oxidant/antioxidant balance was studied by assaying glutathione
(GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and
micronucli frequency. Results: Obesity is associated with enhanced oxidative stress
response. Indeed, vitamin C, E and NADH improved significantly lymphocyte proliferation
and diminished cellular oxidative stress. Conclusion: Treatments of lymphocytes
with vitamins had beneficial effects on lymphocyte proliferation, cytokines secretions
and redox status, generating an anti-inflammatory profile and should be considered
in therapeutic approaches for normalizing immune cell function in obesity.