Determination of External Mass Transfer Model for Hydrolysis of Jatropha Oil Using Immobilized Lipase in Recirculated Packed-Bed Reactor

Chong-Wan Cheng, Rahmath Abdulla, Rao. Rampally Sridhar, Pogaku Ravindra

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DOI: 10.4236/aces.2011.14040                

Abstract

In this study, a simple and effective technique for establishing an external mass transfer model in a recirculated packed-bed batch reactor (RPBBR) with an immobilized lipase enzyme and Jatropha oil system is presented. The external mass transfer effect can be represented with a model in the form of Colburn factor J_D = K Re^-(1–n). The value of K and n were derived from experimental data at different mass flow rates.The experiment shows an average increment of 1.51% FFA for calcium alginate and 1.62% FFA for carrageenan after the hydrolysis took place. Based on different biopolymer material used in immobilized beads, J_D = 1.674 Re^-0.4 for calcium alginate and J_D = 1.881 Re^-0.3 for k-carrageenan were found to be adequate to predict the experimental data for external mass transfer in the reactor in the Reynolds number range of 0.2 to 1.2. The purposed model can be used for the design of industrial bioreactor and scale up. Besides, the external mass transfer coefficients for the hydrolysis of Jatropha oil reaction and the entrapment efficiency for the two biopolymer materials used were also investigated.

Keywords

Pseudomonas Cepacialipase, Jatropha Curcas L. Oil, Carrageenan, Calcium Alginate, Hydrolysis, Packed Bed Reactor, Immobilized Enzyme, External Film Diffusion

Conflicts of Interest

The authors declare no conflicts of interest.

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