TITLE:
Protective Effect of Ischemic Preconditioning on Hepatic Ischemia-Reperfusion Injury in Rats
AUTHORS:
Naoki Hashimoto
KEYWORDS:
Ischemic Preconditioning (IPC), Ischemia/Reperfusion (I/R), Proliferation Index (PI), Reactive Oxygen Species (ROS), Hepatic Ischemia
JOURNAL NAME:
Open Access Library Journal,
Vol.2 No.2,
February
5,
2015
ABSTRACT:
Aim: Ischemic preconditioning (IPC) protects tissue against ischemia and
reperfusion (I/R) injury. The aim of this study was to examine the impact of
IPC on protection of hepatocytes after prolonged I/R injury. Methods: Twenty-four
Wistar rats were randomly divided into ischemic preconditioning group (IP), ischemia/reperfusion
group (IR) and sham operation group (SO). A model of partial liver ischemia/reperfusion
was used, in which rats were subjected to liver ischemia for 90 min prior to
reperfusion. The animals in the IP group underwent ischemic preconditioning for
10 min prior to the ischemia/reperfusion challenge. After 3 h of reperfusion,
serum and liver tissue in each group were collected to detect the level of
serum ALT, AST, HA, MDA and liver histopathology. Results: Compared with IR
group, IP group showed a significantly lower ALT, AST, HA and MDA level in 3 h.
Proliferation index (PI) was significantly increased in IP group compared with
IR group. Conclusion: Ischemic preconditioning can protect liver cells against
ischemia/reperfusion injury, which may be related to cell proliferation during
early ischemic reperfusion.