Susumu Koyama^1,2*, Munechika Enjoji², Mark S. Brodie¹, Sarah B. Appel^1
1Department of Physiology and Biophysics, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.
²Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
DOI: 10.4236/jbise.2015.87040   PDF    HTML   XML   2,905 Downloads   4,049 Views   Citations

Abstract

The ventral tegmental area dopamine (DA VTA) neurons have the spontaneous tonic activity and an alteration of firing pattern from tonic to burst accelerates dopamine transmission more effectively in the mesoaccumbal dopaminergic system, leading to the reinforcing process of drugs of abuse such as alcohol and nicotine. In the present study, we examined whether a persistent Na+ current would contribute to burst firing in DA VTA neuronsusing nystatin-perforated recording. Tetrodotoxin (TTX) (1 μM) or riluzole (10 μM) hyperpolarized the membrane potential and stopped spontaneous firing of DA VTA neurons. In voltage-clamp analysis, a TTX and riluzole-sensitive and persistent Na+ current was activated at ?60 mV and reached maximal amplitude at ?40 mV. This persistent Na+ current was potentiated by a negative shift of the voltage of activation by eliminating Ca²⁺ from the extracellular solution. The Ca²⁺-free extracellular solution depolarized the membrane potential and increased the firing frequency of DA VTA neurons. When a continuous hyperpolarizing current was injected, the firing pattern of the DA VTA neurons transformed into burst-like firing; with average spike number of 4.9, average inter-spike interval of 221 ms, and an average plateau potential, on which the train of spikes generated, was 11 mV. The burst-like firing of DA VTA neurons was abolished by 10 μM riluzole. The concurrent blockade of both T-type Ca²⁺ current and small conductance Ca²⁺-activated K⁺(SK) currents by 100 μM nickel did not induce burst-like firing with or without continuous hyperpolarizing current injection in DA VTA neurons. In conclusion, increases in a persistent Na+ current that mediates a depolarizing driving force by removing extracellular Ca²⁺ contributes to burst-like firing in DA VTA neurons.

Keywords

Apamin, Acutely Dissociated Neurons, Extracellular Ca²⁺, Nickel, Nystatin, Perforated Patch Recording

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1. Introduction

Ventral tegmental area dopamine (DA VTA) neurons project their axons to the nucleus accumbens (NAcb) [1] . Increases in the activity of mesoaccumbal dopamine system in the brain are critical for the reinforcing effects of drugs of abuse, such as ethanol and nicotine [2] - [5] . DA VTA neurons have spontaneous rhythmic activity [6] - [10] and the spontaneous activity of midbrain dopamine neurons regulates dopamine release [11] . DA VTA neurons in vivo exhibit burst firing in addition to regular and irregular firing patterns [12] - [14] and burst firing accelerates dopamine release from dopaminergic nerve terminals more effectively than tonic firing [15] [16] . Therefore, the transformation of tonic firing into burst firing in DA VTA neurons likely contributes to reinforcing processes mediatedvia the mesoaccumbal dopamine system.

The properties of burst firing have been studied in midbrain dopamine neurons in vitro (brain slice preparations) as well as in vivo [17] [18] . Both excitatory synaptic activity and intrinsic ionic mechanisms are considered to contribute to the burst firing of DA VTA neurons. DA VTA neurons receive extensive glutamatergic excitatory synaptic afferents from the prefrontal cortex [19] and the burst firing of midbrain dopamine neurons is induced by the activation of N-methyl-D-aspartic acid (NMDA) receptors on these neurons [18] [20] - [23] . The burst firing of midbrain dopamine neurons also depends on two major ion channels: voltage-dependent Ca²⁺ channels and small conductance Ca²⁺-activated K⁺ (SK) channels. Voltage-dependent L-type Ca²⁺ channels mediate the main depolarizing driving force of spontaneous oscillatory potentials (SOPs), which is around 0.5 Hz, and contribute to the subsequent generation of action potentials (APs) [24] - [26] . A previous study reported that blockade of L-type Ca²⁺ channels prevents carbachol-induced burst firing in DA VTA neurons [27] . It has been reported that the inhibition of SK current induces burst firing in midbrain dopamine neurons [28] [29] . A previous study reported that concurrent blockade of T-type Ca²⁺ channels and SK channels by Ni²⁺ induces burst firing in a subset of midbrain dopamine neurons [30] . Taken together, it is likely that L-type Ca²⁺ current contributes to the depolarizing driving force that induces burst firing and the SK current counters this L-type Ca²⁺ channel-mediated depolarizing driving force to prevent burst firing in midbrain dopamine neurons.

Persistent Na⁺ currents play a critical role in AP generation in neocortical neurons [31] [32] , hippocampal neurons [33] [34] , amygdala neurons [35] , striatal neurons [36] , supraoptic neurons [37] , tuberomammillary neurons [38] , trigeminal neurons [39] , dorsal column neurons [40] and dorsal root ganglion neurons [41] . A recent study has reported that persistent Na⁺ currents contribute to the initiation of AP generation in midbrain dopamine neurons [42] . It is well known that the potentiation of persistent Na⁺ currents induces burst firing in neurons, as demonstrated by experimental procedures such as modulation of Na⁺ channels by phosphorylation [43] - [45] , anemone toxin treatment [46] , or by reduction of extracellular Ca²⁺ concentrations [47] [48] . Therefore, persistent Na⁺ currents may generate burst firing in neurons. In midbrain dopamine neurons, Ca²⁺ current- dependent burst firing has been studied as described above, whereas the properties of Na⁺ current-dependent burst firing have not been well studied. In the present study, we examined the properties of a persistent Na⁺ current and whether this current contributes to burst firing in DA VTA neurons.

2. Materials and Methods

2.1. Perspective of the Experiments

All electrophysiological experiments in the current study were conducted using a patch-clamp experimental system in the Department of Physiology and Biophysics, University of Illinois at Chicago from 2005 to 2006.

2.2. Preparation of Dissociated Neurons

Animals used in this study were treated in strict accordance with the American Physiological Society’s Guiding Principles in the Care and Use of Animals and the U.S. National Institutes for Health Guide for the Care and Use of Laboratory Animals. The protocol for all experimental methods was approved by the Institutional Animal Care Committee of the University of Illinois at Chicago. Fisher rats (F-344; 14 - 18 days old; male and female) were decapitated and the brains quickly removed. The brains were placed in an ice-cold cutting solution (in mM: 220 sucrose, 2.5 KCl, 2.4 CaCl₂, 1.3 MgSO₄, 1.24 NaH₂PO₄, 26 NaHCO₃ and 11_D-glucose), which was constantly bubbled with 95% O₂ and 5% CO₂. Transverse brain slices (400 μm thick) were made on a Vibratome (Series 1000 plus, St. Louis, MO). Brain slices were incubated for 30 min in artificial cerebrospinal fluid (ACSF) (in mM: 126 NaCl, 2.5 KCl, 2.4 CaCl₂, 1.3 MgSO₄ 1.24 NaH₂PO₄, 26 NaHCO₃ and 11 _D-glucose; osmolarity 300 mOsm), which was constantly bubbled with 95% O₂ and 5% CO₂ at room temperature (23˚C - 25˚C). The brain slices were then incubated in a HEPES-buffered external solution (see the next section) containing papain (15 - 18 U/ml) at 32˚C - 36˚C for 20 - 26 min. After papain treatment, the brain slices were further incubated in ACSF for 20 - 40 min. VTA neurons were dissociated with a vibrating stylus apparatus for dispersing cells from the brain slices as previously described [9] . Once the cell dissociation procedure was completed (4 - 7 min), the brain slice was removed from the culture dish, and the dissociated neurons usually settled and adhered to the bottom of the dish within 20 min.

2.3. Nystatin-Perforated Patch Recording

In current-clamp recording, nystatin-perforated patch recording was employed as previously described [8] . Microelectrodes were fabricated on a P-87 puller (Sutter Instrument Company, Novato, CA) from glass capillaries (LE16; Dagan, Minneapolis, MN) and heat-polished on a microforge (Narishige, Tokyo, Japan). The tip resistances of the electrodes were 1.5 - 3 MΩ when filled with pipette solution (in mM: 60 K-acetate, 60 KCl, 1 CaCl₂, 2 MgCl₂ and 40 HEPES; pH adjusted to 7.2 with KOH, final [K⁺]_i = 131 mM; 290 mOsm). Nystatin was dissolved in methanol at a concentration of 10 mg/ml. This nystatin stock solution was diluted with pipette solution to a final concentration of 100 - 200 μg/ml and the electrodes were backfilled with this solution. A HEPES- buffered extracellular solution contained (in mM) 145 NaCl, 2.5 KCl, 2 CaCl₂, 1 MgCl₂, 10 HEPES and 11_D- glucose. The pH was adjusted to 7.4 with NaOH and the osmolarity was 310 mOsm. The HEPES-buffered solution was constantly bubbled with 100% O₂. In some experiments, recording was done in a Ca²⁺-free external solution, in which Ca²⁺ was replaced by equimolar Mg²⁺ to block Ca²⁺ current. The liquid junction potentials between the pipette and extracellular solutions were estimated to be 5 mV [49] and the results have been corrected by this amount. The osmolarity of the solutions was measured by a Vaprovapor pressure osmometer (Wescor Inc., Logan, UT). Electrophysiological measurements were performed using an Axopatch-1B patch clamp amplifier (Axon Instruments, Union City, CA). Membrane currents and voltage were filtered at 1 kHz and acquired at a sampling frequency of 10 kHz. Data acquisition was performed by a DigiData 1322A interface and pClamp software version 9.0 (Axon Instruments). The dissociated VTA neurons were visualized under phase-contrast on an inverted microscope (Diaphot 300, Nikon, Tokyo, Japan). All experiments were performed at room temperature (23˚C - 25˚C).

2.4. Conventional Whole-Cell Recording

In voltage-clamp recording of persistent Na⁺ currents, conventional whole-cell recording was employed with the pipette solution (in mM: 60 Cs-methanesulfonate, 80 CsCl, 5 tetraethylammonium (TEA)-Cl, 4 MgATP, 0.3 Na₂GTP, 5 EGTA and 20 HEPES; pH adjusted to 7.2 with Tris-OH; 310 mOsm). The extracellular solution to record persistent Na⁺ current contained (in mM) 100 NaCl, 40 TEA-Cl, 5 KCl, 2 CaCl₂, 1 MgCl₂, 1 BaCl₂, 1 CsCl, 0.2 CdCl₂, 0.1 NiCl₂, 10 HEPES and 11_D-glucose. The pH was adjusted to 7.4 with Tris-OH and the osmolarity was adjusted to 320 mOsm with sucrose. The extracellular solution was constantly bubbled with 100% O₂. In some experiments, recording was done in a Ca²⁺-free external solution, in which Ca²⁺ was replaced by an equimolar Mg²⁺. The liquid junction potentials between the pipette and extracellular solutions were estimated to be 8 mV and the results have been corrected by this amount.

2.5. Drug Application

Neurons were continuously bathed in the external solution and drugs were dissolved at their final concentration in the same solution. Drug solutions were applied via a multiple channel manifold (MLF-4; ALA Scientific Instruments, Westbury, NY). Each channel of the manifold was connected to a gravity-fed reservoir with tubing (860 μm, i.d.). The output of the manifold was connected to an outflow tube (500 μm, i.d.), the tip of which was placed within 200 μm of the soma of the recorded neuron. Solution flowed continuously through one manifold channel. Application of drug solutions was controlled by opening or closing valves connected to the reservoirs.

2.6. Source of Drugs and Chemical Agents

The following drugs and chemical agents were used in this study, apamin, BaCl₂, CsCl, CdCl₂, EGTA, HEPES, nystatin, riluzole, TEA-Cl and tetrodotoxin (TTX) were purchased from Sigma?Aldrich (Saint Louis, MO). Papain was purchased from Worthington (Lakewood, NJ). Riluzole was dissolved in dimethyl sulphoxide (DMSO) and made as a stock solution at a concentration of 100 mM. The stock solution for riluzole was diluted in the HEPES-buffered external solution. The final concentration of DMSO in the HEPES-buffered external solution was 0.01%.

2.7. Data Analysis

Action potentials (APs) were analyzed offline with pClamp 9.0 software (Axon Instruments Inc.). To assess the change of spontaneous firing with drugs, a 60-s-long segment during the peak of the drug response was analyzed as previously described [50] . Inter-spike interval (ISI) histograms were created as previously described [51] . The number of bins was equal to the square root of the number of ISIs. Bin width was obtained by dividing the ISI range (maximum ISI minus minimum ISI) by the number of bins. The coefficient of variation (CV) of the events was obtained by dividing the standard deviation of the event intervals by the mean event intervals. Burst duration was measured between the first spike and the last spike of a spike train. The ISI during burst-like firing was obtained by dividing the burst duration by the number of spike intervals in the burst spike train. The plateau potential, on which the trains of spikes were generated, was measured from the bottom of membrane potential to the threshold of the first spike. The data from cells with AP amplitudes less than 50 mV were discarded. All average values are expressed as mean ± standard error of the mean (S.E.M.). Statistical comparison to assess significant differences was done by the paired or unpaired Student’s t-test. To analyze persistent Na⁺ current activation, the current was normalized to the maximal current and fitted by the Boltzmann equation, using Origin software version 7 (Origin Lab, Northampton, MA):

where y is the current at the test voltage of x, A₁ is the minimal current and set to be 0, A₂ is the maximal current and set to be 1, x₀ is the membrane potential for half-activation and dx is the slope factor.

3. Results

3.1. Na⁺ Current Contribution to Spontaneous Firing of DA VTA Neurons

Figure 1(A) shows typical spontaneous membrane activity before, during, and after the application of 1 μM TTX in DA VTA neurons. Before TTX treatment, the DA VTA neuron exhibited spontaneous firing (Figure 1(A)-a). TTX hyperpolarized the membrane potential and abolished spontaneous firing (Figure 1(A)-b). After the washout of TTX, AP generation reappeared (Figure 1(A)-c). Figure 1(B) shows typical spontaneous membrane activity before, during, and after the application of 10 μM riluzole, a voltage-dependent Na⁺ channel antagonist, in DA VTA neurons. Before riluzole treatment, the DA VTA neuron exhibited spontaneous firing (Figure 1(B)-a). Riluzole hyperpolarized the membrane potential and abolished spontaneous firing (Figure 1(B)-b). After the washout of riluzole, AP generation reappeared (Figure 1(B)-c).

3.2. TTX-and Riluzole-Sensitive Persistent Na⁺ Currents

To ascertain the presence of persistent Na⁺ currents in DA VTA neurons, we used a ramp-voltage protocol with potassium-based pipette solutions and physiological extracellular solutions Figure 2(A₁) shows typical currents before and after treatment with 1 μM TTX and TTX-sensitive currents were obtained by the digital subtraction of TTX-insensitive currents from control currents. Figure 2(B₁) shows typical currents before and after treatment with 10 μM riluzole and riluzole-sensitive currents were obtained by the digital subtraction of the riluzole-insensitive currents from control currents. The riluzole-sensitive inward current provided a similar current-

voltage relationship to the TTX-sensitive current. Figure 2(C) shows the current density-voltage relationship of riluzole-sensitive currents in DA VTA neurons.

3.3. Potentiation of Persistent Na⁺ Currents with Ca²⁺-Free Extracellular Solution

We next examined the current-voltage relationship of persistent Na⁺ current by a voltage-step protocol using cesium-based pipette solution. To identify DA VTA neurons, cell-attached recording was performed before making conventional whole-cell configuration. In the 6 DA VTA neurons examined, average spontaneous firing frequency was 1.5 ± 0.2 Hz. Figure 3(A₁) shows currents induced by the voltage-step protocol before and after application of 2 μM TTX in the control extracellular solution. Figure 3(A₂) shows currents induced by the voltage-step protocol before and after application of 2 μM TTX in the Ca²⁺-free extracellular solution. The graph in

Figure 3(B) shows the current-voltage relationship of TTX-sensitive persistent Na⁺ currents in the control extracellular solution and the Ca²⁺-free extracellular solution. Figure 3(C) shows the normalized persistent Na⁺ current plotted as a function of test voltage before and after the elimination of Ca²⁺ from the extracellular solutions. In the control extracellular solution, average half-activating voltage (V_{act, 0.5}) was −53.6 ± 0.7 mV and average slope factor was 3.4 ± 0.2 (n = 6). In the Ca²⁺-free extracellular solution, average V_{act, 0.5} was −57.1 ± 0.7 mV and average slope factor was 3.7 ± 0.2 (n = 6). The elimination of Ca²⁺ from the extracellular solution significantly shifted the V_{act, 0.5} in a negative direction (P < 0.001) without changing the slope.

3.4. Na⁺ Current Contribution to Burst-Like Firing

We examined the effect of Ca²⁺-free extracellular solution, which potentiated persistent Na⁺ current, on the firing of DA VTA neurons. Figure 4(A₁) shows typical membrane activity before, during, and after the application of Ca²⁺-free extracellular solution with or without continuous hyperpolarizing current injection. In the control extracellular solution, the spontaneous firing frequency was 1.6 Hz (Figure 4(A₂), a). The Ca²⁺-free extracellular solution depolarized the membrane potential and increased the firing frequency to 7.6 Hz (Figure 4(A₂), b). When a continuous hyperpolarizing current was injected to counter the depolarizing driving force, burst-like firing was generated in the Ca²⁺-free extracellular solution (Figure 4(A₂), c). When the continuous hyperpolarizing current injection was removed and the Ca²⁺-free extracellular solution was switched to the control extra-

cellular solution, the firing frequency became 1.7 Hz (Figure 4(A₂), d). Figure 4(B) shows APs in control and burst-like firing in a faster time scale. In the burst-like firing, a train of spikes rode on a plateau potential and the spike amplitude gradually decreased during the spike train (Figure 4(B)). As shown in Figure 4(C), the firing patterns before and after burst-like firing were analyzed by ISI histograms. The ISI is normally distributed with a peak at 590 ms in control (Figure 4(C₁), while the ISI histogram skewed leftward with a wider ISI distribution during the burst-like firing (Figure 4(C₂). In 11 neurons out of 16 DA VTA neurons (69%), burst-like firing was generated in the Ca²⁺-free extracellular solution, when continuous hyperpolarizing current was injected (−48.5 ± 5.6 pA); the average CV of firing frequency was 1.62 ± 0.17. The burst-like firing parameters were as follows: burst duration, 826.5 ± 105.2 ms; spike number in the train, 4.9 ± 0.2; ISI, 221.2 ± 18.9 ms; plateau potential, 10.7 ± 0.6 mV (n = 11). As illustrated in Figure 4(D), the burst-like firing induced by the continuous hyperpolarizing current injection in the Ca²⁺-free extracellular solution was abolished after treatment with 10 μM riluzole; average membrane potential after treatment with riluzole was −62.3 ± 1.4 mV (n = 4).

3.5. Blockade of T-Type Ca²⁺ Channels and SK Channels Did Not Induce Burst Firing

In Ca²⁺-free extracellular solution, reduced Ca²⁺ influx through voltage-dependent Ca²⁺ channels decreases Ca²⁺- activated K⁺ currents. If this occurred in DA VTA neurons, the counter effect of SK currents would oppose decreases in the depolarizing driving force and burst firing may be induced as previously described in midbrain dopamine neurons [28] [29] . To represent the condition of blocking both voltage-dependent Ca²⁺ current and SK current in Ca²⁺-free extracellular solution, we used nickel to produce concurrent blockade of T-type Ca²⁺ channels and SK channels, since these two ion channels are strongly coupled in midbrain dopamine neurons [30] . We ascertain that blockade of SK channels by 200 n Mapamin reduced AHP in DA VTA neurons (Figure 5(A)). In

a similar manner, Ni²⁺ (100 μM) reduced AHP, which is attributable to SK currents (Figure 5(B)). Before nickel treatment, the spontaneous firing frequency was 1.7 Hz (Figure 5(C₂), a). The firing frequency was decreased and firing pattern became irregular in the presence of 100 μM Ni²⁺ (Figure 5(C₂), b). When a continuous hyperpolarizing current was injected during nickel treatment, the firing frequency was decreased and abolished without inducing burst firing (Figure 5(C₂), c). After the continuous hyperpolarizing current injection was removed and Ni²⁺ was washed out, the spontaneous firing frequency became 1.6 Hz (Figure 5(C₂), d). In 5 DA VTA neurons, continuous hyperpolarizing current injection with nickel treatment failed to generate burst firing; the average continuous hyperpolarizing current was −20.0 ± 3.5 pA.

4. Discussion

In our voltage-clamp analysis, the persistent Na⁺ current, which was TTX- and riluzole-sensitive, was activated at −60 mV and reached a maximal amplitude at −40 mV in DA VTA neurons. Previous studies have reported that TTX- and riluzole-sensitive persistent Na⁺ currents are activated at membrane potentials positive to −60 mV in suprachiasmic nucleus neurons [52] , dorsal column nucleus neurons [40] , and neocortex neurons [53] . In our current-clamp analysis, blockade of Na⁺ current with TTX or riluzole caused membrane hyperpolarization and blocked AP generation in DA VTA neurons. Our findingsindicate that persistent Na⁺ current is activated at subthreshold membrane potentials and has a critical role in AP generation in DA VTA neurons, supporting the findings of a previous study in midbrain dopamine neurons [42] .

In the present study, the Ca²⁺-free extracellular solution potentiated persistent Na⁺ currents by a negative shift of the activation voltage by 3.5 mV without changing the slope factor, indicating that persistent Na⁺ current in the Ca²⁺-free extracellular solution can be activated by negative membrane potentials between −63.5 mV and −43.5 mV. It is known that extracellular Ca²⁺reductions potentiate persistent Na⁺ currents and this contributes to burst firing [46] [47] . In our current study, burst-like firing induced by the Ca²⁺-free extracellular solution was completely prevented with 10 μM riluzole, suggesting that the Na⁺ current is a major depolarizing driving force in these neurons. We found that concurrent blockade of both T-type Ca²⁺ channels andSK channels by 100 μM Ni²⁺ did not induce burst-like firing in DA VTA neurons. In the presence of nickel, continuous hyperpolarizing current of −20 pA was able to stop AP generation completely. The burst-like firing of DA VTA neurons in Ca²⁺- free extracellular solution may be induced by a different mechanism from the reduction of SK currents. This is confirmed by comparing properties of Na⁺ current-dependent burst-like firing in the current study and those of burst firing induced by the inhibition of SK channels with apamin [28] . The Na⁺ current-dependent burst-like firing in the present study had short-term spindle-like plateau with small number of APs during the burst, whereas burst firing induced by SK channel inhibition has long-term ramp-like plateau with greater number of APs during the burst [28] .

It is useful to compare the properties of Na⁺ current-dependent burst-like firing observed in the present study and Ca²⁺-dependent carbachol-induced burst firing in DA VTA neurons [27] . For Na⁺ current-dependent burst- like firing parameters, the average burst duration was 827 ms, average spike number per burst was 4.9, and average ISI within the burst was 221 ms. Conversely, L-type Ca²⁺ current-dependent burst firing induced by carbachol has a longer burst duration (5 - 10 s), a larger number of spikes (more than 10 during the burst) and a longer ISI within the burst (599 ms) [27] . Another difference between Na⁺ current-dependent and Ca²⁺-depen- dent carbachol-induced burst firings is a difference in plateau potentials: a spindle-like plateau potential in Na⁺ current-dependent burst-like firing and a slowly depolarizing plateau-like potential which lasts for 5 - 10 s in Ca²⁺ current-dependent burst firing [27] . In midbrain dopamine neurons, burst firing induced by the activation of NMDA receptors [22] and by blockade of SK channels with the activation of metabotropic glutamate receptors [29] have similarities with the Ca²⁺ current-dependent burst firing induced by carbachol [27] . Na⁺ current-de- pendent burst-like firing in the present study has similarities with findings of a previous study using brain slice preparations, in which average spike number per burst is 3.7 and average ISI within the burst is 73 ms [18] .

A previous study has reported that the concurrent blockade of both T-type Ca²⁺ currents and SK currents with 100 μM Ni²⁺ induced burst firing in a subset of midbrain dopamine neurons [30] , whereas 100 μM Ni²⁺ failed to induce burst firing in the present study. The differences between our findings and those of previous studies may depend on experimental preparations, brain slices, and acutely dissociated neurons. In midbrain dopamine neurons from brain slice preparations, Ca²⁺ current-mediated SOPs (0.5 - 1.0 Hz), which underlie the generation of spontaneous firing, are prominent and spontaneous Ca²⁺ spikesare generated by Na⁺ current blockade in some neuronal populations [24] [25] . In dissociated DA VTA neurons, the amplitude of the SOPs was not prominent (<5 mV) (Figure 1), suggesting that the Ca²⁺ current contribution to the depolarizing driving force is small in these dissociated neurons. In brain slice preparations, it is likely that Ca²⁺ currentscontribute to AP generation and Na⁺ currents supportAP generation. In addition, SK currents counter the Ca²⁺ current-mediated depolarizing driving force to prevent midbrain dopamine neurons from burst firing in brain slice preparations.

In the present study, the burst-like firing was induced in the dissociated DA VTA neurons under the blockade of Ca²⁺ channels and the elimination of presynaptic effects on these neurons, however, there is a potential limitation in the experimental design of the study. The burst firing was induced by a non-physyological maneuver such as the elimination of extracellular Ca²⁺ with hyperpolarizing current injection. Our findings present a possible biophysiological model for burst firing in DA VTA neurons and suggest that both Na⁺ current- and Ca²⁺ current-dependent intrinsic ionic mechanisms contribute to burst firing in these neurons. Future work will be necessary to delineate the physiological contribution of these ionic currents to burst firing that increases dopamine transmission more effectively in the mesoaccumbal regions in association with accelerated mediation of reinforcing process.

Acknowledgements

This work was supported by National Institute on Alcohol Abuse and Alcoholism Grant AA05846 (to S.B.A.). This study was partly supported by Grants-in-Aid for Scientific Research (C) (Nos. 22500685 and 25350166 to S.K.) from the Japan Society for the Promotion of Science (JSPS) and the Mishima Kaiun Memorial Foundation, Japan (to S.K.). This study was also supported by funds (No. 106006 to S.K.) from the General Research Institute of Fukuoka University.

Competing Interests

The authors have no competing interests to declare.

NOTES

^*Corresponding author.

Conflicts of Interest

The authors declare no conflicts of interest.

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