TITLE:
Induction of DnaK upon γ-Irradiation at High and Low Rates in Escherichia coli
AUTHORS:
Linda Saucier, Claudine Dube, Anthony Guernec, Fadia Naim
KEYWORDS:
DnaK; Chaperone; Irradiation; Bacterial Stress Response; Efficacy of Antimicrobials
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.3 No.10,
October
12,
2012
ABSTRACT: DnaK is implicated in protein folding, repair and degradation. Its protective role during heat shock is well documented and many other stresses can also induce its production. Using a competitive ELISA, intracellular DnaK concentrations were determined in Escherichia coli ATCC 25922 exposed to a γ-irradiation dose of 0.3 KGy applied either at high (8 × 10-2 KGy/min) or low rates (3 × 10-3 KGy/min) and with or without a recuperation period of 22 h at 37℃ post-treatment. All four irradiation treatments reduced cell counts similarly and significantly compared to the control (P