TITLE:
Assessment of the Quality of Frozen-Thawed Semen or Epididymal Sperm in Three Native Vietnamese Pig Breeds
AUTHORS:
Van Khanh Nguyen, Huong Thu Thi Vu, Huong Le Thi Nguyen, Huong Thi Nguyen, Au Thi Hoang, Dat Van Le, Nhung Tuyet Thi Nguyen, Hieu Trung Phan, Yen Kim Thi Pham, Son Phan Le, Quynh Anh Le, Quynh Anh Le, Lan Doan Pham, Minh Quang Luu, Muoi Thi Nguyen
KEYWORDS:
Native Vietnamese Pig, In Vitro Porcine Embryo, Epididymal, Ejaculate, Artificial Insemination
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.13 No.3,
July
12,
2023
ABSTRACT: Our aim was to evaluate the quality of
ejaculated and epididymal frozen-thawed pig sperm of endangered Vietnam native pig breeds.
Ejaculated sperm was collected from live boars and epididymal sperm was
collected from slaughtered boars of the MuongTe, Kieng Sat and Co BinhThuan breeds and frozen in 0.25 ml straws using a protocol established earlier for
modern pig breeds. We evaluated the sperm quality after thawing in terms of
motility and rates of viable and abnormal spermatozoa. Our results revealed
that the sperm motility and rates of viable and
abnormal frozen-thawed sperm were >30%, >44%, and in
vitro. In the Co BinhThuan breed, ejaculated sperm generated higher
cleavage, blastocyst and hatching rates than did the epididymal sperm
(60.11% vs 56.02%, 17.23% vs 14.31%, 3.78% vs 2.34%,
respectively, P , the hatching blastocyst rate was different between the breeds (P > 0.05). In the Co BinhThuan breed, the rate of pregnancy of ejaculated groups was similar to
that of the epididymal group.
In conclusion, the
ejaculated and epididymal sperm of native Vietnamese pigs were successfully frozen. We succeeded in creating embryos in vitro and pregnant pigs after artificial insemination from frozen-thawed
semen in three native Vietnamese pig breeds for the first time. The use of the
ejaculated sperm improved the production of native pig embryos in vitro efficiency.