TITLE:
IgA1 from HSP Patients Trigger Apoptosis and Inhibit Cytoskeletal Proteins in HUVEC
AUTHORS:
Liping Yuan, Wenjun Fei, Lin Wu, Ming Gui, Qin Zhang, Bo Hu
KEYWORDS:
Henoch-Schönlein Purpura (HSP); Endothelial Cells; Immunoglobulin A; Apoptosis; Cytoskeletal Proteins
JOURNAL NAME:
Open Journal of Pediatrics,
Vol.4 No.1,
March
6,
2014
ABSTRACT:
Background: Henoch-Schonlein purpura (HSP) is a
kind of systemic small vessel vasculitis in children. Endothelium cells injury
induced by IgA1 is considered important in the pathogenesis of HSP. Research
found that the apoptosis of vein endothelial cells was related to the
vasculitis in HSP patients. Purpose:
To observe the effect of IgA1 from HSP patients on the apoptosis of HUVEC and
firstly analyze the mechanism of the apoptosis of HUVEC induced by IgA1.
Methods: HUVECs were cultured in 3 different conditional
media with IgA1 from HSP patients, normal healthy children and simply medium
(blank control). Serum IgA1 was purified by jacalin affinity chromatography.
The rates of apoptosis in HUVEC incubated with IgA1 were determined by TUNEL
method and flow cytometry, respectively. The expression of the cytoskeletal
proteins, such as FAK, Vinculin and MLCK was detected with the methods of
Real-time PCR and Westernblot, respectively. Results: The present study showed
that the apoptosis rate of HUVEC by IgA1 isolated from HSP patients was higher than blank control (14.77% ±
2.23% vs 2.25% ± 0.77%) (P on IgA1
from HSP patients may induce apoptosis of vascular endothelial cells through
inhibiting the cytoskeletal proteins expression. IgA1 may accelerate
progression of HSP by inducing apoptosis of vascular endothelial cells.