TITLE:
Persian Shallot (Allium hirtifolium Boiss) Extract Elevates Glucokinase (GCK) Activity and Gene Expression in Diabetic Rats
AUTHORS:
Mehdi Mahmoodi, Sadegh Zarei, Mohsen Rezaeian, Mohammad Kazemi Arababadi, Hassan Ghasemi, Hossein Khoramdelazad, Naser Rezayati, Gholamhossein Hasanshahi, Seyed-Mostafa Hosseini-Zijoud
KEYWORDS:
Persian Shallot; Glucokinase; Gene Expression; Diabetes
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.7,
July
5,
2013
ABSTRACT:
Hepatic GCK is a key enzyme in glucose homeostasis and, as such, is a
potential target for treatment strategies of diabetes. We investigated the
effect of Persian shallot (Allium hirtifolium Boiss) hydroalchoholic
extract on blood glucose level, plasma insulin level, GCK activity and its gene
expression. Thirty two male rats
were divided into 4 groups of 8, diabetic groups received 100 and 200 mg/kg
Persian shallot extract, diabetic control and normal control received 0.9%
saline for 30 days. Investigations of gene expression by Real-Time PCR showed
that Persian shallot had led to gently increased GCK gene expression in diabetic rats. GCK activity
increased significantly in Persian shallot treated group in dose dependent
manner (P