TITLE:
Multiplex Rapid Test with Acceptable Diagnosis Performance as a Solution to Increase Diagnosis of Hepatitis B and C Viruses in Pregnant Women in an Area of High Prevalence of Both Hepatitis Viruses Associated with HIV
AUTHORS:
Catherine Boni-Cisse, Nina Esther Onstira Ngoyi, Hermione Dahlia Mossoro-Kpinde, Noella Packo, Rabi Senekian, Hermann Ndoidet Koutou, Coretha Bokia-Baguida, Christelle Luce Bobossi-Gadia, Arthur Simplice Sombot-Ndicky, Daniel Yvon Gonessa, Freddy Samuel Ngbonga Konzapa Voulou, Christian Diamant Mossoro-Kpinde
KEYWORDS:
HIV-HCV-HBV Co-Infection, Multiplex Immunochromatographic Rapid Test, Central Africa, Serology Test Cost, Diagnostic Accessibility
JOURNAL NAME:
Open Journal of Medical Microbiology,
Vol.14 No.1,
March
28,
2024
ABSTRACT: Background and Objective: HIV, hepatitis B virus (HBV) and hepatitis C virus
(HCV) are very widespread in the world, however, less than 20% of the
people affected are diagnosed and treated. This study aimed to determine the
prevalence of HIV, HCV and HBV co-infections in pregnant women at Bangui
Community University Hospital and the cost of screening. Methods: A
cross-sectional study involving consenting pregnant women who came for
antenatal care was performed. HIV, HCV antibodies and HBV antigens were detected using Exacto Triplex? HIV/HCV/HBsAg rapid test,
cross-validated by ELISA tests.
Sociodemographic and professional data, the modes of transmission and
prevention of HIV and both hepatitis viruses were collected in a standard sheet
and analyzed using the Epi-Info software version 7. Results: Pregnant
women aged 15 to 24 were the most affected (45.3%); high school girls (46.0%), and pregnant women living in cohabitation (65.3%) were the most represented.
Twenty-five (16.7%) worked in the formal sector, 12.7% were unemployed housewives and the remainder in the
informal sector. The prevalence of HIV, HBV, and HCV viruses was 11.8%, 21.9%
and 22.2%, respectively. The prevalence of co-infections
was 8.6% for HIV-HBV, 10.2% for HIV-HCV, 14.7% for HBV-HCV and 6.5% for
HIV-HBV-HCV. All positive results and
10% of negative results by the rapid test were confirmed by ELISA tests. The
serology of the three viruses costs 39,000 FCFA (60 Euros) by ELISA compared to
10,000 FCFA (15.00 Euros) with Exacto Triplex? HIV/HCV/AgHBs (BioSynex,
Strasbourg, France). Conclusion: The low level of education and
awareness of hepatitis are barriers to development and indicate the importance
of improving the literacy rate of women in the Central African Republic (CAR). Likewise, the high prevalence of the
three viruses shows the need for the urgent establishment of a national program
to combat viral hepatitis in the CAR.