TITLE:
Quantitative Evaluation of Inflammatory Response in Rats Submitted to the Abdominal Wall Surgery Using Peritoneum of Paca Preserved in 300% Supersaturated Solution of Sucrose or 98% Glycerin
AUTHORS:
Leonardo Martins Leal, Alessandra Regina Scavone Ferreira, Tais Harumi de Castro Sasahara, Aliny Antunes Barbosa Lobo Ladd, Paola Castro Moraes, Márcia Rita Fernandes Machado
KEYWORDS:
Healing, Pathology, Graft, Wild, Stereology
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.8 No.4,
October
29,
2018
ABSTRACT: This study aimed to quantitative
evaluate the implantation of paca’s peritoneum, a new biomaterial option,
preserved in 300% supersaturated sugar solution or 98% glycerin on the
abdominal wall of Wistar rats. 60 males, weighing between 150 and 200 g, are organized
into the following experimental groups: control group (GI); group peritoneum
preserved in 300% supersaturated sugar solution (GII); and group peritoneum
preserved in 98% glycerin (GIII). Each group had 20 animals. Five rats from
each group underwent euthanasia at four different moments: 7th, 15th,
30th, and 60th post-operatory day for histological and
stereological evaluations of the graft/native tissue interface. On histological
examination, one found out an extensive presence of inflammatory infiltrate at
the early periods (7th and 15th day) and an extensive
presence of connective tissue at the late moments (30th and 60th day). Through stereological analysis, one found out that the number of
mononuclear cells decreased throughout the evaluation moments. One concluded
that the paca’s peritoneum as a biological membrane preserved in 98% glycerin,
when implanted on the abdominal wall of rats, microscopically allowed one to
obtain, through stereological analyses, a lower inflammatory response than the
paca’s peritoneum preserved in 300% supersaturated sugar solution.