TITLE:
Detection of Promoter Hypermethylation of GSTP1 and CDH1 Genes and the Relationship of Histopathological Parameters of the Breast
AUTHORS:
Onur Eroglu, Mine Erci Baysak, Beyhan Durak Aras, Oguz Cilingir, Sevilhan Artan
KEYWORDS:
Breast Cancer, Methylation, GSTP1, CDH1, Histopathological Types
JOURNAL NAME:
Advances in Breast Cancer Research,
Vol.7 No.2,
March
15,
2018
ABSTRACT: Background: Breast cancer is the most common cancer in women. Histopathology plays an
important part in determining the treatment strategy for women with breast cancer.
GSTP1 plays an important role in protecting cells from cytotoxic and carcinogenic
agents and it is expressed in normal tissues at variable levels in different cell
types. CDH1 plays a critical role for establishment and maintenance of polarity
and differentiation of epithelium during the development period. Also, it
plays an important role in signal transduction, differentiation, gene expression,
cellmotility and inflammations. Methods: In this study the promoter methylation
levels of GSTP1 and CDH1 gene which are associated with breast cancer were investigated
by technique of Methylation Sensitive High Resolution Melting Analysis (MS-HRM).
We analysed primary tumor core biopsies from 80 high-risk primary breast cancer
patients (tumors ≥ 2 cm and/or lymphatic metastase and/or distant metastases and/or
under 40 years). Also the patients’ histopathologic
types were associated with the methylation levels. Results: In our study
the promoter hypermethylation status was observed
at different rates; GSTP1 and CDH1 hypermethylation frequencies were 82% and 95%
respectively. The promoter hypermethylation levels of the genes were found to be significant with lymph node positivity, ER positivity and HER2/neu
negativity. Conclusion: Our study is important as being the first study that
analyzes association between histopathologic type and GSTP1 and CDH1 gene promotor
methylation status in Turkish population.