TITLE:
Evaluation of Allelochemicals, Abscisic Acid and Coumarin, in Leaf-Origin Suspension Cultured Cells of Prunus yedoensis Using Protoplast Co-Culture Bioassay Method
AUTHORS:
Kouhei Fujise, Shinso Yokota, Hamako Sasamoto
KEYWORDS:
Allelopathy, Bioassay, Protoplast Culture, Prunus Species
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.9 No.2,
January
24,
2018
ABSTRACT: Dried
leaves of Prunus yedoensis and P. lannesiana (50 mg) showed
strong inhibitory allelopathic activities, e.g., more than 97% growth
inhibition of lettuce seedling using the sandwich method. Similarly, among suspension cultures induced from leaves and peduncles of two Prunus species, we found the strongest inhibitory allelopathic activities of protoplasts of leaf-origin suspension cells
of P. yedoensis, when the
protoplast co-culture method for bioassay of allelopathy was applied with
lettuce as a recipient plant. Effects of two putative allelochemicals, abscisic
acid and coumarin, on both protoplast cultures of lettuce and P. yedoensis were investigated. Coumarin inhibited the growth of lettuce protoplasts from low concentrations, while abscisic acid
stimulated. Abscisic acid inhibited the protoplast growth of P.
yedoensis from low
concentrations, while coumarin did not, but inhibited only at a high
concentration (1 mM). Contents of abscisic acid in protoplasts were measured
using small scale purification and Enzyme Linked Immno Sorbent Assay, and
contents of coumarin in leaf-origin
susepension cells of P. yedoensis were measured using Gas
Chromatography-Mass Spectrometry. Coumarin was more likely the allelochemical
causing the strong inhibitory allelopathic activities of P. yedoensis in
the protoplast co-culture bioassay. Effectiveness of the protoplast co-culture
bioassay method of allelopathy was discussed.