TITLE:
Effect of Alteration of Glutathione Content on Cell Viability in α-Synuclein-Transfected SH-SY5Y Cells
AUTHORS:
Ken-Ichi Tanaka, Kanako Sonoda, Masato Asanuma
KEYWORDS:
α-Synuclein, L-Buthionine-SR-Sulfoximine, N-Acetyl-L-Cysteine, Glutathione, SH-SY5Y Cells, Parkinson’s Disease
JOURNAL NAME:
Advances in Parkinson's Disease,
Vol.6 No.3,
August
23,
2017
ABSTRACT: It is well known that α-synuclein (αS) plays an important role in the pathogenesis
of Parkinson’s disease (PD). Moreover, oxidative stress is also thought to
be an important factor in PD due to induction of dopaminergic neuronal cell
death by free radicals and enhancement of αS fibrillation by oxidized stress. In
the present study, to clarify the role of glutathione (GSH), an intracellular antioxidant,
on the molecular mechanism of αS-induced cell injury, we examined
the effects of L-buthionine-SR-sulfoximine (BSO), a GSH synthase
inhibitor, with or without N-acetyl-L-cysteine (NAC), a source of GSH, on
αS-induced cell injury in human neuroblastoma SH-SY5Y cells. Treatment with
BSO significantly reduced the cell viability of both empty-vector- and αS-transfected
SH-SY5Y cells in a dose-dependent manner (p