TITLE:
Application of Glyceroglycolipids, Photosynthetic Pigments and Extracts of Brown Algae for Suppression ROS
AUTHORS:
Natalia Gerasimenko, Ekaterina Menchinskaya, Andrey Esipov, Dmitry Aminin, Stepan Logvinov, Eugene Pislyagin
KEYWORDS:
Algae, Glyceroglycolipids, Fucoxanthin, Fatty Acids, Reactive Oxygen Species (ROS)
JOURNAL NAME:
Open Journal of Marine Science,
Vol.6 No.3,
July
5,
2016
ABSTRACT: Lipid-rich extracts were
obtained from brown algae Saccharina cichorioides, Costaria costata, Chorda filum, Eularia fistulosa, Dictyopteris divaricata, Dictyosiphon
chordaria, Silvetia
babingtonii, and Fucus evanescens that were collected in Peter the
Great Gulf of the Sea of Japan. The ability of algalextracts and glyceroglycolipids (GLs)
monogalactosyldiacylglycerols (MGDG), digalactosyldiacylglycerols (DGDG), and
sulfoquinovosyldiacylglycerols (SQDG) and carotenoid fucoxanthin to suppression of reactive oxygen
species (ROS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage
cells, studied. The results showed that algae extracts could suppress ROS. However, extracts of D. divaricata, D. chordaria, C. filum, S. babingtonii, and F.
evanescens had a higher
degree of suppression of ROS. Extracts
of S. cichorioides and D.
divaricata showed the
dependence of their activity from the month of collecting these algae. The GLs
and fucoxanthin were isolated from extracts of using column chromatography with
silica gel and their ROS-inhibitory activity was investigated too. The fatty
acids (FAs) composition of lipids was determined byGC and GC/MS. It has been found that MGDG and DGDG stronger than SGDG
inhibited the ROS and the degree of their activity depended on the species of
algae, the month of collection, the amount of PUFA, the ratio of n - 3 and n - 6 PUFA in GLs. Fucoxanthin has shown a high degree of
suppression of ROS. This preliminary study has shown the
prospect of a deeper study of the suppression of ROS with the help of lipids
from algae the Sea of Japan.