TITLE:
Isolation and Characterization of Flavonols by HPLC-UV-ESI-MS/MS from Talipariti elatum S.w
AUTHORS:
José González Yaque, Armando Cuéllar, Lionel Massi, Max Monan, Enmanuel Nossin, Frantz François-Haugrin
KEYWORDS:
Flavonoids, HPLC, MS, Petals, Chemical Composition
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.8,
June
9,
2016
ABSTRACT: The red petals of the flowers of Talipariti
elatum, commonly named majagua is used as antitussive, expectorant and
antasthmatic in phytotherapy, although the plants’ composition has not been determined
in detail so far. Hence, in this study, we present a validated, sensitive,
reliable, and cheap narrow-bore LC-UV-ESI-MS/MS coupled to PDA (photodiode
array detectors) method for the simultaneous isolation and identification of
flavonoids and their glycosidic derivatives in this flower drug. In addition,
the structures of two compounds have been elucidated by LC-MS experiments after
isolation. Structure analyses allow proposing the presence of gossypitrin
(gossypetin-7-O-glucoside) or
gossypetin-3'-O-glucoside, a
quercetin derivative, possibly quercetin-3-O-glucoside
and an unidentified compound with an impair number of m/z, probably an alkaloid.