TITLE:
High Frequency Plant Regeneration from Leaf Derived Callus of Dianthus caryophyllus L.
AUTHORS:
Muhammad Arif, Saima Rauf, Aziz Ud Din, Mamoona Rauf, Humera Afrasiab
KEYWORDS:
Dianthus caryophyllus L., Regeneration Potential, Plant Growth Regulators, Morphogenic Callus Induction
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.15,
July
29,
2014
ABSTRACT:
An efficient
procedure was developed for in vitro callus induction, proliferation and regeneration of carnation cultivar (Dianthus caryophyllus L.) using leaf,
nodal and inter-nodal explants on Murashige and Skoog’s medium (MS)
supplemented with exogenous plant growth regulators. For morphogenic callus
induction and proliferation from various explants, MS medium supplemented with
3.0 mg/l 2,4-D was highly efficient with 100% callus induction frequency from
inter-nodal explants. Leaf explants showed quicker response than nodal and
inter-modal explants, for callus initiation within 6 days of inoculation. Best
grown callus was obtained from leaf explant. The leaf-derived callus was
maintained up to several weeks, which indicated that 8-week incubation period
was the most suitable for obtaining well proliferated, morphogenic callus.
Temperature variation also affected the growth of in vitro induced morphogenic callus from various explants. Results
have shown that 27°C proved to be the best temperature for morphogenic callus
induction and proliferation from leaf and inter-nodal explants. Among the
auxin-cytokinin combination, MS medium containing 1.0 mg/l
N(6)-benzylaminopurin (BAP) and 2.0 mg/l NAA showed the highest efficiency of
callus initiation and proliferation from leaf, nodal and inter-nodal explants.
Light conditions proved better for callogenesis and proliferation from leaf,
nodal and inter-nodal explants. Regeneration response from well grown
morphogenic callus was prominent on MS medium supplemented with 3.0 mg/l BAP
alone and 1.0 mg/l NAA with 3.0 mg/l BAP.