TITLE:
Optimization of Quantitative Analysis of Naproxin Sodium Using UV Spectrophotometery in Different Solvent Mediums
AUTHORS:
Muhammad Hashim Zuberi, Urooj Haroon, Yasmin BiBi, Talat Mehmood, Iffat Mehmood
KEYWORDS:
Naproxen Sodium; UV Spectrophotometry; Pharmaceutical Analysis
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.5 No.3,
February
26,
2014
ABSTRACT:
A new rapid, simple and reproducible UV spectrophotometric method was developed and validated for the
estimation of Naproxen Sodium (NpSd) in bulk and pharmaceutical formulation.
The quantification of NpSd was done at 230 nm in methanol and in buffer of pH
6.8 and 9. Beer’s law was obeyed in the
concentration range of 4 - 36 (r2 = 0.999)
in methanol and 5 - 25 μgmL﹣1 in buffer of pH 6.8 and 9 (r2 = 0.988 and 0.997) respectively. The apparent molar absorptivity values were
also calculated in all mediums. All parameters according to ICH guideline were
tested and validated. The detection and quantitation limits were found to be
0.054, 0.083, 0.073 and 0.181, 0.251, 0.211 μgmL﹣1 respectively. These methods were applied directly to the analysis of the
pharmaceutical tablet preparations (Anex? tablet 250 mg). The
results demonstrated that the procedure is accurate, precise and reproducible
(relative standard deviation 3%), while being simple, cheap and
less time consuming and hence can be suitably applied for the estimation of
NpSd in dosage forms and dissolution studies.