TITLE:
In-Situ Synthesized Myoglobin Imprinted Poly(N-Isopropylacrylamide)/Silica Nanocomposite Gels by Using the Molecular Imprinting Method
AUTHORS:
Burcu Meşhur, Eylem Turan, Nursel Dilsiz
KEYWORDS:
Nanocomposite Gels; Poly(N-Isopropylacrylamide); Temperature Sensitive; Myglobin; Free Radical Crosslinking Polymerization; Adsorption; Imprinted
JOURNAL NAME:
Advances in Materials Physics and Chemistry,
Vol.4 No.1,
January
15,
2014
ABSTRACT:
Temperature
sensitive imprinted poly(N-isopropylacrylamide) nanocomposite gels were syntheses
via in-situ, free radical
crosslinking polymerization of corresponding monomer in nano-sized silica and
five different concentrations of myoglobin solution by using the molecular
imprinting method. Mb adsorption from five different concentrations of Mb
solutions was investigated by two types of nanocomposite gel systems prepared
by non-imprinted and imprinted methods. Nanocomposite gels imprinted with Mb
showed higher adsorption capacity and specificity for Mb than nanocomposite
gels prepared by the usual procedure. The highest Mb adsorption was observed via
the imprinted nanocomposite gels with 12.5% Mb. In addition, selectivity studies were also performed by using
two reference molecules as fibrinogen and hemoglobin. The imprinted
nanocomposite gels had higher adsorption capacity for Mb than the non-imprinted
gels and also exhibited good selectivity for Mb and high adsorption rate
depending on the number of Mb sized cavities.