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has been cited by the following article:

• TITLE: Rapid Detection Method for Enteroaggregative Escherichia coli Using Simple Clump Formation and Aggregative Assay

  AUTHORS: Miyuki Fujioka, Chowdhury Rafiqul Ahsan, Yoshimitsu Otomo

  KEYWORDS: Escherichia coli; Aggregative Adherence; Clump Formation; Rapid Detection

  JOURNAL NAME: Advances in Microbiology, Vol.3 No.8, December 17, 2013

  ABSTRACT: Enteroaggregative Escherichia coli (EAggEC) strains cause the persistent diarrhea in infants and compromised hosts in developing countries. These strains are currently defined as E. coli that adheres to HEp-2 cells in an aggregative adherence (AA) pattern. In this study, we compared 4 different rapid methods for the detection of EAggEC using a PCR assay, clump formation test, glass slide adherence assay, and the HEp-2 cell adherence assay. Out of 683 E. coli strains isolated from diarrheal stool samples, we detected 17 aggR and/or clump-positive strains, and identified 2 aggR-positive, clump-negative strains and 2 aggR-negative, clump-positive strains. All the aggR positive and clump positive strains also showed positive results in glass slide adherence and HEp-2 cell adherence assays. From all these results, we suggest the following procedure for the rapid identification of EAggEC strains: first, screen E. coli strains with the clump formation test and subsequently perform the glass slide adherence assay to observe AA for confirmation.