Photo-Induced DNA-Dependent Conformational Changes in Silver Nanoparticles

Vasil G. Bregadze, Zaza G. Melikishvili, Tamar G. Giorgadze

Department of Biological System Physics, Ivane Javakhishvili Tbilisi State University, Elevter Andronikashvili Institute of Physics, Tbilisi, Georgia.
Department of Coherent Optics and Electronics,Georgian Technical University, Vladimir Chavchanidze Institute of Cybernetics, Tbilisi, Georgia.

DOI: 10.4236/anp.2013.22026                

Abstract

The goal of the present investigation is to study the interaction of AgNPs with thymus DNA by traditional and original spectra-photometric and thermo-dynamic methods and approaches in darkness and under photo-irradiation. 1) At the interaction of DNA with nano-particles hypsochromic shift of 6 nm and hypochromic effect of 20% of AgNPs absorption band are observed. 2) At photo-irradiation (λ = 436 nm or full spectrum of visible band) of AgNPs-DNA complexes absorption spectra band width is changed from 140 nm to 360 nm at half-height. Besides, isosbestic point is observed. 3) Kinetic study of photo-diffusion has made it possible to determine desorption rate constant and desorption reaction activation energy that are equal respectively to k_d @ 9 ′ 10^-5 s^-1, E_d @ 80 kJ/M Ag⁰ for AgNPs bound with DNA. It is shown that AgNPs represent liquid drops which moisture the DNA surface at interaction. At photo-irradiation of AgNPs-DNA complex DNA dependant conformational transition takes place due to fast and intensive heating.

Keywords

Component; DNA-Silver Nanoparticle Complexes; Optical Spectroscopy of Intermolecular Interactions;Photo-Dynamic Effect; Silver Nanoparticles; Silver Ions

Conflicts of Interest

The authors declare no conflicts of interest.

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