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has been cited by the following article:

• TITLE: Determination of 17α-Methyltestosterone in Freshwater Samples of Tilapia Farming by High Performance Liquid Chromatography

  AUTHORS: Isabel R. Barbosa, Sara Lopes, Rhaul Oliveira, Inês Domingues, Amadeu M. V. M. Soares, António J. A. Nogueira

  KEYWORDS: 17α-Methyltestosterone; High Performance Liquid Chromatography; Environment; Tilapia Farming

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.4 No.4, April 29, 2013

  ABSTRACT: 17α-methyltestosterone is used to induce the sex reversal of Tilapia sp. to obtain cultures mono-sex to an economically viable. This practice may lead to environmental contamination and problems in human health. Therefore methods need to be developed to detect residues of 17α-methyltestosterone in aqueous matrices. A simple high-performance liquid chromatographic method using ultraviolet detection (245 nm) and testosterone as internal standard has been developed for the monitoring 17α-methyltestosterone in freshwater samples of tilapia aquaculture. The method described involves limited sample preparation as it includes a filtration followed by a single solid-phase extraction step using C18 cartridge. Validation data indicated that the HPLC-UV method for 17α-methyltestosterone determination in the concentration range of 50 - 2000 μg/L provided good linearity, sensitivity, accuracy and precision. Method performance was efficiently applied to monitoring the freshwater samples of fish ponds and the surrounding aquatic channels.