TITLE:
Vaccination against urinary tract infections caused by E. coli
AUTHORS:
Hartwig Wilhelm Bauer, Hira Shams, Ricarda Michaela Bauer
KEYWORDS:
Pyelonephritis; Outer Membrane Antigen; Iron Compound Receptors
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.3A,
March
29,
2013
ABSTRACT:
Uropathogenic Escherichia coli (UPEC) causes uncomplicated
urinary tract infection (UTI) depicts a prevalent and potentially uncompromising
infectious disease. In this analysis, we explained the functions of an immunoproteomics
concept to vaccine development that has been successfully employed to recognize
vaccine targets in other pathogenic bacteria. Pyelonephritis strains E. coli CFT073 are used for outer membrane
isolation mimics urinary tract environment in which iron limitation, osmotic stress,
human urine, and exposure to uroepithelial cells are included. During experiments
of UTI, the antigens that induce the humoral immune response is to identified, two-dimensional
gel electrophoresis are employed for the isolation of outer membrane protein and
probed using pooled antisera from 20 CBA/J mice chronically infected with E. coli CFT073. 23 total outer membrane
antigens, in which a unique iron compound receptor is included, are reacted with
antisera and were identified by mass spectrometry. These antigens comprises of
proteins with known functions in UPEC pathogenesis such as, ChuA, IroN, IreA, Iha,
IutA, and FliC. These all information and data elaborate that these factors are
associated with virulence during UTI are directed by antibody response. We also
represents that the genes encoding ChuA, IroN, hypothetical protein c2482, and IutA
are significantly more prevalent among UPEC strains than among fecal-commensal E. coli isolates.
Therefore we concluded that, the outer membrane
antigens are identified in this study are conserved, could be reflective
part for the UTI vaccine generated to induce protective immunity against UPEC infections.