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has been cited by the following article:

• TITLE: Protective Effects of Polyphenol Extracts from Sea Buckthorn (Hippophaë rhamnoides L.) on Rat Hearts

  AUTHORS: Lin Tang, Henghui Lv, Shu Li, Hongna Bi, Xin Gao, Jianhua Zhou

  KEYWORDS: Hippophaë rhamnoides L., Polyphenol Extracts, Myocardial Ischemia Reperfusion Injury, Autophagy, Antioxidant Activity

  JOURNAL NAME: Open Journal of Molecular and Integrative Physiology, Vol.6 No.1, February 22, 2016

  ABSTRACT: The objective of present study was to investigate the antioxidative and cardioprotective effects of polyphenol extracts from sea buckthorn (PESB) against myocardial ischemia reperfusion injury (MIRI). PESB was analyzed using HPLC-DAD and their antioxidant activities were evaluated by measuring the intrinsic free radicals cavenging capacity and inhibiting the formation of hydroxyl radical capacity. Experiments on the isolated rat hearts were carried out according to the improved Langendorff retrograde perfusion technology. Coronary effluent of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) activities were analyzed to determine the extent of myocardial injury. Protein expression levels of Beclin-1, microtubule-associated protein 1 light chain 3 (LC3) I and II were measured to evaluate the degree of autophagy. In conclusion, coupled results from both in vivo and in vitro experiments have confirmed that PESB has potential health implications in both prevention and amelioration of MIRI, which may be attributed to the inhibition of autophagy.